葛花醇提液对离体蛙心收缩功能的影响  

Effects of Ethanol Extract of Pueraria lobata(Willd)Ohwi(EEPL)on Systolic Function of Isolated Frog Hearts

作　　者：谭娟 向德标 袁祎玲 徐梦婷 TAN Juan;XIANG De-biao;YUAN Yi-ling;XU Meng-ting(School of Biological and Food Engineering,Huaihua University;Key Laboratory of Research and Utilization of Ethnomedicinal Plant Resources of Hunan Province;Key Laboratory of Hunan Higher Education for Hunan Xiangxi medicinal Plant and Ethnobotany,Huaihua,Hunan 418008)

机构地区：[1]怀化学院生物与食品工程学院 [2]民族药用植物资源研究与利用湖南省重点实验室 [3]湘西药用植物与民族植物学湖南省高校重点实验室,湖南怀化418008

出　　处：《怀化学院学报》2020年第5期1-5,共5页Journal of Huaihua University

基　　金：湖南省教育厅一般项目“葛花醇提物对血脂代谢异常大鼠调节作用及其机理研究”(16C1274);湖南省重点实验室委托项目“不同来源的葛藤总黄酮和葛根素含量比较及其抗氧化研究”(YYZW2015-156).

摘　　要：为了探讨葛花对心脏活动的影响,也为进一步发掘葛花的药理作用提供理论依据,本实验采用斯氏蛙心插管法制备离体蛙心,用BL-420F生物机能实验系统观察记录不同浓度葛花醇提液对离体蛙心收缩活动的影响,并采用受体阻断剂探讨葛花醇提液的作用机制.结果表明:葛花醇提液对离体蛙心收缩活动具有“双向调节”作用,在0.5 mg/mL^5 mg/mL浓度范围内,表现为促进作用,在2.5 mg/mL时最显著(P<0.01);当浓度达到10 mg/mL时开始表现为抑制作用,50 mg/mL时抑制作用最显著(P<0.01).其机制可能与α肾上腺能受体和M受体有关.To investigate the effects of Effects of Ethanol Extract of Pueraria lobata(EEPL)on hearts and provide theoretical basis for pharmacological action on further exploration on Pueraria lobata,We prepared isolated frog hearts using Straw method in this experiment,record the effects of EEPL on the isolated frog hearts by BL-420F biological signal recording system,and discuss the mechanism of EEPL using receptor blocker.The results show that the EEPL demonstrates“bidirectional regulation”effects on the isolated frog hearts.Between the concentration range of 0.5 mg/mL^5 mg/mL,the EEPL promoted the contractile performance of isolated frogs,where the most significant was at 2.5 mg/mL(P<0.01).At 10 mg/mL,it shows inhibitory effect,and 50 mg/mL has the most significant inhibitory effects(P<0.01).Its action mechanism may be related to adrenergicαreceptor and cholinergic M receptor.

关 键 词：葛花醇提液 离体蛙心 收缩力 Α受体 M受体 

分 类 号：R285[医药卫生—中药学]