超声提取-高效液相色谱法测定土壤中氨基苯磺酸的方法研究  

Study on Ultrasonic Extraction-High Performance Liquid Chromatography Method for Determination of Aminobenzene Sulfonic Acids in Soil

作　　者：李光明 LI Guang-ming(CCIC Physical and Chemical Testing Co.Ltd.,Shanghai 200436,China)

机构地区：[1]中检集团理化检测有限公司,上海200436

出　　处：《环境监控与预警》2020年第6期27-31,共5页Environmental Monitoring and Forewarning

摘　　要：建立了超声提取-高效液相色谱法测定土壤中氨基苯磺酸的方法。以甲醇/水(V∶V=1∶1)为萃取溶剂,室温下进行超声波提取,通过固相萃取小柱净化后,以带季铵基团的聚乙烯醇离子色谱柱分离,二极管阵列检测器测定。该实验方法具备较好的抗干扰性,方法曲线相关系数r>0.999,取样量为5 g时,对、间、邻氨基苯磺酸的方法检出限分别为0.1,0.2和0.2 mg/kg,加标回收率分别为83.5%~99.5%,83.1%~98.0%和77.8%~92.5%。对5个地区的土壤样品加标回收实验结果表明,该方法适用性较强,可满足实际样品的测定。An Ultrasonic Extraction-High Performance Liquid Chromatography method for the determination of aminobenzene sulfonic acids in soil sample was established.Using methanol-water(V∶V=1∶1)as extraction solvent,ultrasonic extraction was performed at room temperature,sample extract was cleaned up by solid phase extraction cartridge,separated by a polyvinyl alcohol ion chromatographic column bonded with quaternary ammonium group,and analyzed by diode array detector.The method showed good anti-interference performance,the linear correlation coefficient r>0.999,and the method detection limit ofρ-Aminobenzene sulfonic acid was 0.1 mg/kg,m-Aminobenzene sulfonic acid was 0.2 mg/kg while o-Aminobenzene sulfonic acid was 0.2 mg/kg when sample weight was 5 g.The recoveries were 83.5%~99.5%,83.1%~98.0%and 77.8%~92.5%,respectively.The results of the recovery experiment of soil samples in 5 regions showed that the method has strong applicability and can meet actual needs.

关 键 词：高效液相色谱 土壤 对氨基苯磺酸 间氨基苯磺酸 邻氨基苯磺酸 

分 类 号：X833[环境科学与工程—环境工程] O657.7+2[理学—分析化学]