天龙通心片对犬急性心肌缺血损伤的影响  

Effect of Tianlong Tongxin Tablet on Acute Myocardial Ischemia Injury in Dogs

作　　者：李玲美 李磊[1] 付建华[1] 史跃[1] 马彦雷[1] 刘建勋[1] LI Ling-mei;LI Lei;FU Jian-hua;SHI Yue;MA Yan-lei;LIU jian-xun(Institute of Basic Medical Sciences of Xiyuan Hospital,China Academy of Chinese Medical Sciences,Beijing 100091,China)

机构地区：[1]中国中医科学院西苑医院基础医学研究所,北京100091

出　　处：《中国实验方剂学杂志》2020年第23期92-98,共7页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81774145,81503292);国家“重大新药创制”科技重大专项(2012ZX09103201-049);国家重点基础研究发展计划课题(2015CB554406)。

摘　　要：目的:观察天龙通心片口服给药对实验性犬急性心肌缺血及相关指标的影响。方法:建立犬急性心肌缺血模型,分为正常组(等量β-环糊精10 g·kg-1),合心爽组(5 mg·kg-1),天龙通心片高、中、低剂量组(1,0.5,0.25 g·kg-1),复方丹参片组(0.144 g·kg-1)。采用心外膜电图标测心肌缺血程度,定量组织学(N-BT染色法)测定心肌梗死范围,测定冠脉血流量、心输出量、心肌耗氧量、冠脉阻力及外周阻力,同时用光学试剂盒检测血清肌酸激酶(CK),乳酸脱氢酶(LDH)及血清超氧化物歧化酶(SOD)活性,丙二醛(MDA)含量。结果:与正常组比较,天龙通心片能减小由心外膜电图标测的心肌缺血程度(∑-ST)(P<0.05),缩小经N-BT染色所显示的梗死区(P<0.05),降低静脉血氧含量等作用(P<0.05),增加麻醉犬冠脉流量、心输出量及心肌耗氧量,降低冠脉阻力及外周阻力(P<0.05),而对血清CK,LDH及血清SOD活性,MDA含量的影响未见统计学差异。结论:天龙通心片具有一定的改善犬急性心肌缺血和心肌梗死的作用。Objective: To observe the effect of oral administration of Tianlong Tongxin tablet on acute myocardial ischemia and related indexes in experimental dogs. Method: The model of acute myocardial ischemia in dogs was established and the dogs were divided into the control group(equal amount of normocyclodintrin 10 g·kg-1),Hexinshuang group(5 mg·kg-1),Tianlong Tongxin tablet high,medium and low dose groups(1,0.5,0.25 g·kg-1)and the compound Danshen tablet group(0.144 g·kg-1). Myocardial ischemia degree was measured by epicardium electrocardiogram,the range of myocardial infarction was determined by quantitative histology(N-BT staining), and coronary blood flow, cardiac output, myocardial oxygen consumption,coronary resistance and peripheral resistance were measured. Meanwhile,serum creatine kinase(CK),lactate dehydrogenase(LDH)and serum superoxide dismutase(SOD)activity and malondialdehyde(MDA)content were detected by optical kit. Result:As compared with the control group,Tianlong Tongxin tablet can reduce the myocardial ischemia degree(∑-ST)measured by the electrocardiogram of the pericardium(P<0.05),reduce the infarcted area shown by N-BT staining(P<0.05),reduce the venous oxygen content(P<0.05),increase the coronary flow,cardiac output and myocardial oxygen consumption of anesthetized dogs,and reduce the coronary artery resistance and peripheral resistance(P<0.05). However,there was no significant difference in the influence of serum CK, LDH, SOD activity and MDA content in serum.Conclusion:Tianlong Tongxin tablet can improve acute myocardial ischemia and myocardial infarction in dogs.

关 键 词：天龙通心片 急性心肌缺血 犬 

分 类 号：R2-0[医药卫生—中医学] R22