苏云金杆菌与印楝素对水稻二化螟的灭杀协同增效作用研究  

Synergistic Effect of Bacillus thuringiensis and Azadirachtin against Chilo suppressalis

作　　者：金党琴[1] 田连生[1] 龚爱琴[1] 王珍 吴耀月 JIN Dangqin;TIAN Liansheng;GONG Aiqin;WANG Zhen;WU Yaoyue(Yangzhou Polytechnic Institute,Yangzhou 225127,China)

机构地区：[1]扬州工业职业技术学院,江苏扬州225127

出　　处：《云南农业大学学报（自然科学版）》2020年第6期963-967,共5页Journal of Yunnan Agricultural University:Natural Science

基　　金：扬州市重点研发计划项目(YZ2018049);江苏高校“青蓝工程”(苏教师[2018]12号);江苏省第五期“333高层次人才培养工程”(苏人才[2016]7号,BRA2017479);江苏省高等学校优秀科技创新团队(苏教科〔2017〕6号);江苏省自然科学基金项目(BK20181219)。

摘　　要：【目的】以水稻二化螟为供试害虫,对苏云金杆菌(Bacillus thuringiensis,Bt)与印楝素协同增效作用进行研究。【方法】采用Bt预处理和直接混用2种处理方式,测定2种处理对二化螟的活性并进行田间药效试验。【结果】经Bt预处理后24和48 h后,二化螟的LC50值分别为1.15和0.42 mg/L,增效作用明显且优于直接混用。经Bt预处理后,印楝素对二化螟的田间防治效果明显,施药后14 d杀虫效果为91.20%、保苗效果为94.64%、枯心率为0.23%。【结论】Bt预处理对印楝素增效作用明显,优于两者单独使用和直接混用,为防治水稻二化螟新型生物农药的研发提供依据。[Purpose]The synergetic effect of Bacillus thuringiensis(Bt)and azadirachtin was studied by using rice stem borer as the test insect.[Method]Two kinds of treatments,Bt pretreatment and direct mixture,were used to measure the synergistic effect against Chilo suppressalis,and the field control experiment was carried out.[Result]After Bt pretreatment,the LC50 values of C.suppressalis were 1.15 mg/L and 0.42 mg/L after 24 h and 48 h,respectively,and the synergistic effect was obvious and better than the direct mixture.After Bt pretreatment,the effect of azadirachtin on the field control of C.suppressalis was significant.The insecticidal effect was 91.20%,the seedling-preserving effect was 94.64%,and the heart-burning rate was 0.23%after 14 days of application.[Conclusion]After Bt pretreatment,the synergistic effect on azadirachtin is obvious,which is better than the two alone or direct mixture,which provides a basis for the development of new biological pesticides for the control of C.suppressalis.

关 键 词：苏云金杆菌 印楝素 二化螟 增效作用 

分 类 号：S482.292[农业科学—农药学]