转LeNCED1基因对白三叶ABA含量及水分利用效率的影响  

Effect of LeNCED1 Gene on the ABA Content and Water Use Efficiency in White Clover

作　　者：王昌耀 闫尔俊 常凯 宗亚倩 鲁洪智 周凯 姜华[1] WANG Changyao;YAN Erjun;CHANG Kai;ZONG Yaqian;LU Hongzhi;ZHOU Kai;JIANG Hua(College of Animal Science and Technology of Yunan Agricultural University,Kunming 650201,China;Party School of Wuding County Committee of the Communist Party of China,Wuding 651500,China)

机构地区：[1]云南农业大学动物科学技术学院,云南昆明650201 [2]中共武定县委党校,云南武定651600

出　　处：《云南农业大学学报（自然科学版）》2020年第6期1029-1033,共5页Journal of Yunnan Agricultural University:Natural Science

基　　金：云南省重点研发计划课题(2018BB002-02);云南省技术创新人才(2018HB075)。

摘　　要：【目的】研究LeNCED1基因对白三叶水分利用效率的影响,为白三叶的节水抗旱提供一定的理论指导。【方法】分别以7株含转SAG13-LeNCED1基因白三叶和非转基因白三叶的老叶和嫩叶为材料,研究其ABA的含量、光合特性、叶绿素含量及水分利用效率。【结果】转SAG13-LeNCED1基因显著提高了白三叶老叶的ABA含量(P<0.05),却显著降低了白三叶老叶的叶绿素b、胞间CO2浓度、蒸腾速率和气孔导度(P<0.05)。转LeNCED1基因显著降低了白三叶嫩叶叶绿素a、叶绿素b、净光合速率和蒸腾速率(P<0.05),却显著提高了白三叶嫩叶的胞间CO2浓度和气孔导度(P<0.05)。【结论】转入LeNCED1基因能显著提高白三叶的长期水分利用效率(P<0.05)。[Purpose]The effect of the LeNCED1 gene on the water use efficiency of white clover was studied to provide a certain theoretical guidance for its water saving and drought resistance.[Method]We studied the ABA content,photosynthetic characteristics,pigment and water use efficiency of the old and young leaves in the transgenic(LeNCED1)plants with the SAG13 promoter and non-transgenic white clover.[Result]The LeNCED1 gene could significantly increase the ABA content in the old leaves of white clover(P<0.05),but reduced significantly chlorophyll b,intercellular CO2 concentration,transpiration rate and stomatal conductance in the old leaves of white clover(P<0.05).What’s more,the LeNCED1 gene decreased the content of chlorophyll a,chlorophyll b,net photosynthesis rate and transpiration rate in young leaves of white clover(P<0.05),but significantly increased the intercellular CO2 concentration and stomatal conductance of young leaves of white clover(P<0.05).[Conclusion]The long-term water use efficiency of white clover was improved by transferring LeNCED1.

关 键 词：白三叶 LeNCED1基因 脱落酸 水分利用效率 

分 类 号：S541.201[农业科学—作物学]