HPLC法测定活力苏口服液中迷迭香酸、紫草酸、丹酚酸B、淫羊藿苷、宝藿苷I、二苯乙烯苷和虎杖苷  

Determination of rosmarinic acid, lithospermic acid, salvianolic acid B, icariin, baohuoside Ⅰ, 2,3,5,4’-tetrahydroxystilbene-2-O-β-D-glucoside, and polydatin in Huolisu Oral Liquid by HPLC

作　　者：段光霞 牛小萍 程红杰 DUAN Guang-xia;NIU Xiao-ping;CHENG Hong-jie(Department of Pharmacy,Zhengzhou Yihe Hospital,Zhengzhou 450047,China;Department of Pharmacy,The Fifth Affiliated Hospital of Zhengzhou University,Zhengzhou 450006,China;Anyang Center for Food and Drug Control,Anyang 455000,China)

机构地区：[1]郑州颐和医院药学部,河南郑州450047 [2]郑州大学第五附属医院药学部,河南郑州450006 [3]安阳市食品药品检验检测中心,河南安阳455000

出　　处：《现代药物与临床》2020年第10期1950-1953,共4页Drugs & Clinic

摘　　要：目的建立高效液相色谱法同时测定活力苏口服液中迷迭香酸、紫草酸、丹酚酸B、淫羊藿苷、宝藿苷I、二苯乙烯苷和虎杖苷。方法采用Agilent Eclipse XDB-C18色谱柱(250 mm×4.6 mm,5μm),流动相为乙腈–0.1%磷酸溶液,梯度洗脱,检测波长280 nm,体积流量1.0 mL/min,柱温30℃,进样量10μL。结果迷迭香酸、紫草酸、丹酚酸B、淫羊藿苷、宝藿苷I、二苯乙烯苷和虎杖苷分别在0.59~14.75、0.81~20.25、5.07~126.75、1.86~46.50、1.18~29.50、8.79~219.75、2.07~51.75μg/m L线性关系良好(r≥0.9991);各成分平均加样回收率分别为96.95%、97.80%、99.43%、98.53%、99.19%、100.01%、98.77%,RSD值分别为1.13%、1.28%、0.91%、0.88%、0.97%、0.62%、1.33%。结论所建立的方法操作便捷,结果准确,为全面评价活力苏口服液的内在产品质量提供了科学依据。Objective To establish an HPLC method for simultaneous determination of rosmarinic acid, lithospermic acid, salvianolic acid B, icariin, baohuoside Ⅰ, 2,3,5,4’-tetrahydroxystilbene-2-O-β-D-glucoside and polydatin in Huolisu Oral Liquid. Methods Agilent Eclipse XDB-C18 column(250 mm × 4.6 mm, 5 μm) was used for the separation. The mobile phase was consisted of acetonitrile-0.1% phosphoric acid solution with gradient elution at a flow rate of 1.0 mL/min. The column temperature was 30 ℃, and the detection wavelength were set at 280 nm. Results Rosmarinic acid, lithospermic acid, salvianolic acid B, icariin, baohuoside Ⅰ, 2,3,5,4’-tetrahydroxystilbene-2-O-β-D-glucoside and polydatin had good linear relationships in the ranges of 0.59 — 14.75, 0.81 — 20.25, 5.07 — 126.75, 1.86 — 46.50, 1.18 — 29.50, 8.79 — 219.75, and 2.07 — 51.75 μg/m L(r ≥ 0.999 1). The average recoveries of components were 96.95%, 97.80%, 99.43%, 98.53%, 99.19%, 100.01%, and 98.77% with the RSD values of 1.13%, 1.28%, 0.91%, 0.88%, 0.97%, 0.62%, and 1.33%, respectively. Conclusion The established method is convenient and accurate, which provides a scientific basis for the comprehensive evaluation of product quality of Huolisu Oral Liquid.

关 键 词：活力苏口服液 迷迭香酸 紫草酸 丹酚酸B 淫羊藿苷 宝藿苷I 二苯乙烯苷 虎杖苷 高效液相色谱 

分 类 号：R286.02[医药卫生—中药学]