皮下植入戊二醛固定的牛心包对大鼠免疫功能的影响  

Effect of subcutaneous embedding of glutaraldehyde-fixed bovine pericardium on the immunologic function of rats

作　　者：吴昊 李芹 龚德军 刘晓红 徐志云 WU Hao;LI Qin;GONG Dejun;LIU Xiaohong;XU Zhiyun(Department of Cardiovascular Surgery,Shanghai Changhai Hospital,Shanghai 200433,China)

机构地区：[1]上海长海医院心血管外科,上海200433

出　　处：《中华实用诊断与治疗杂志》2020年第11期1086-1088,共3页Journal of Chinese Practical Diagnosis and Therapy

基　　金：国家重点研发计划(2016YFC1100900)。

摘　　要：目的观察大鼠皮下包埋经戊二醛固定的牛心包组织后脾脏系数、胸腺系数等指标变化,探讨其对大鼠免疫系统的影响。方法SD大鼠8只,随机分为观察组和对照组各4只。观察组皮下包埋经体积分数0.2%戊二醛固定的牛心包组织,对照组仅行手术操作但不包埋牛心包组织。观察2组大鼠皮下包埋后精神状态、进食饮水情况、伤口感染发生情况;2组分别于包埋前及包埋后28d称体质量,计算体质量增长率;处死大鼠取脾脏和胸腺组织,称质量后计算脾脏、胸腺系数;取部分脾脏组织制备细胞悬液,采用CCK-8法检测T淋巴细胞、B淋巴细胞增殖;取剩余脾脏、胸腺组织行组织病理检查。结果皮下包埋结束约20min,2组大鼠精神恢复正常,观察期内饮食和饮水正常,伤口恢复良好,无感染等症状。观察组大鼠脾脏系数[(2.60±0.20)mg/g]大于对照组[(1.78±0.43)mg/g](P<0.05),体质量增长率[(0.78±0.08)%]低于对照组[(1.03±0.14)%](P<0.05),胸腺系数[(2.17±0.22)mg/g]与对照组[(1.95±0.25)mg/g]比较差异无统计学意义(P>0.05)。观察组T淋巴细胞刺激指数(0.19±0.02)与对照组(0.21±0.08)比较差异无统计学意义(P>0.05),B淋巴细胞刺激指数(0.98±0.10)高于对照组(0.23±0.08)(P<0.05)。观察组脾脏白髓较对照组轻度扩大,红髓内可见轻度水肿淤血,2组胸腺区域未见明显差异。结论经戊二醛固定的牛心包可激活大鼠的体液免疫系统。Objective To observe the changes of the spleen and thymus indexes after subcutaneous embedding with glutaraldehyde-fixed bovine pericardium,and to investigate its influence on immune system in rats.Methods Eight SD rats were randomly and equally divided into observation group and control group.The rats in observation group were subcutaneously embedded with 0.2%glutaraldehyde-fixed bovine pericardial tissue,and the rats in control group received operation but no embedding.After subcutaneous embedding,the mental state,eating and drinking water status and wound infection were observed.The body mass was weighed before and 28dafter embedding in two groups to calculate the growth rate.The rats were killed to weigh the spleen and thymus respectively to calculate the spleen and thymus indexes.Partial spleen tissues were taken to prepare cell suspension,and the proliferations of T lymphocytes and B lymphocytes were detected by CCK-8 method.The remained spleen and thymus tissues received histopathological examination.Results The mental state of rats in two groups returned normal in 20min after embedding,the rats had normal diet and drinking water,and the wound healed well without infection.The spleen index was larger in observation group((2.6±0.20)mg/g)than that in control group((1.78±0.43)mg/g)(P<0.05),and the growth rate of body mass was significantly lower than that in control group((0.78±0.08)%vs.(1.03±0.14)%)(P<0.05).There was no significant difference in the thymus index between two groups((2.17±0.22)mg/g vs.(1.95±0.25)mg/g)(P>0.05).There was no significant difference in the stimulation index of T lymphocytes between observation group and control group(0.19±0.02 vs.0.21±0.08)(P>0.05).The stimulation index of B lymphocytes was significantly higher in observatisn group(0.98±0.10)than that in control group(0.23±0.08)(P<0.05).The pathological results showed that the white medulla of the spleen in observation group was slightly enlarged than that in control group,while mild edema and congestion were found in the

关 键 词：免疫系统 牛心包 戊二醛 皮下包埋 大鼠 

分 类 号：R654.2[医药卫生—外科学]