锌掺杂二氧化硅在雪菊中木犀草苷分析中的应用  

Application of zinc-doped silica for determination of luteoloside in the Coreopsis tinctoria

作　　者：韩想 阿迪拉·阿布都热西提 贺小刚 冯昱龙 楚刚辉 HAN Xiang;ABUDUREXITI Adila;HE Xiaogang;FENG Yulong;CHU Ganghui(Xinjiang Laboratory of Native Medicinal and Edible Plant Resources Chemistry,College of Chemistry and Environmental Science, Kashgar University, Kashgar 844000, China)

机构地区：[1]新疆药食用植物资源化学重点实验室喀什大学化学与环境科学学院,新疆喀什844000

出　　处：《功能材料》2020年第11期11214-11220,共7页Journal of Functional Materials

基　　金：国家自然基金资助项目(21765012);新疆维吾尔自治区高校科研计划自然科学重点资助项目(XJEDU2020I016)。

摘　　要：采用简易的方法制备了一种锌掺杂二氧化硅吸附剂,建立了一种通过近红外漫反射光谱快速选择性测定雪菊中木犀草苷的分析方法。在讨论对吸附剂吸附率的影响条件中,对pH值、吸附剂的用量和吸附时间进行了研究。用近红外光谱方法对富集了木犀草苷的吸附剂直接进行检测,并且在对模型的定量校正过程中使用了偏最小二乘法。以上研究发现,在中性条件下常温吸附20 min吸附剂质量为0.25 g时,木犀草苷的吸附率可达到87.1%;经SNV处理后,木犀草苷校正模型的参考浓度和预测浓度两者之间的相关系数是0.9909,预测回收率在87%~118%范围内。结果表明,锌掺杂二氧化硅吸附剂可以有效地富集雪菊中微量的木犀草苷,将近红外光谱和化学计量学方法相结合实现了雪菊中微量的木犀草苷快速、灵敏、选择性地测定。这对于其他药食两用植物中微量活性成分的检测提供了参考依据。A zinc-doped silica adsorbent was prepared by simple method,and an analytical method for rapid and selective detection of luteoloside in Coreopsis tinctoria was established by near-infrared diffuse reflectance spectroscopy.During the discussion of the influence conditions on the adsorption rate of the adsorbent,the pH value,the dosage of adsorbent and the adsorption time were studied.The adsorbent enriched with luteoloside was detected directly by near infrared spectroscopy,and a quantitative correction model was established by partial least square method.The analytical method of near-infrared spectroscopy found that the adsorption rate of luteoloside reached 87.1%when the adsorbent mass was 0.25 g for 20 min at room temperature under neutral conditions.After SNV treatment,the correlation coefficient between the reference concentration and the predicted concentration of luteoloside correction model was 0.9909,and the predicted recovery was in the range of 87-118%.The results showed that the zinc-doped silica adsorbent could effectively enrich the trace amount of luteoloside in Coreopsis tinctoria.The near-infrared spectroscopy combined with stoichiometric method realized the rapid,sensitive and selective determination of trace amount of luteoloside in Coreopsis tinctoria.This provided a reference for the detection of trace active components in other medicinal plants.

关 键 词：锌掺杂二氧化硅吸附剂 木犀草苷 定量校正 富集 近红外光谱 

分 类 号：R284.2[医药卫生—中药学]