Ⅱ型固有淋巴样细胞(ILC2)通过分泌IL-13减缓类风湿性关节炎患者的发病进程  

Group 2 innate lymphoid cells (ILC2) relieves pathogenesis of rheumatoid arthritis by secreting IL-13

作　　者：李鑫 吴刚 金向楠 苗小青 王亚媛 刘畅 马思扬 沈晖[3] LI Xin;WU Gang;JIN Xiangnan;MIAO Xiaoqing;WANG Yayuan;LIU Chang;MA Siyang;SHEN Hui(Department of Rheumatology,First Affiliated Hospital of Jinzhou Medical University,Jinzhou 121001;Department of Breast Surgery,First Affiliated Hospital of Jinzhou Medical University,Jinzhou 121001;Department of Rheumatology,First Affiliated Hospital of China Medical University,Shenyang 110000,China)

机构地区：[1]锦州医科大学附属第一医院风湿免疫科,辽宁沈阳110000 [2]锦州医科大学附属第一医院乳腺外科,辽宁沈阳110000 [3]中国医科大学附属第一医院风湿免疫科,辽宁沈阳110000

出　　处：《细胞与分子免疫学杂志》2020年第9期815-820,共6页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金(81373219);辽宁省教育厅基金(jc2019009)。

摘　　要：目的研究Ⅱ型固有淋巴样细胞(ILC2)在类风湿性关节炎(RA)中的变化及可能的机制。方法收集RA患者进行28个关节疾病活动度评分(DAS28),并收集外周血标本。全自动血沉器检测血沉(ESR)、免疫透射浊度法检测C反应蛋白(CRP)。免疫比浊法检测类风湿因子(RF)、化学发光法检测抗环瓜氨酸肽抗体(ACCP)。流式细胞术检测RA患者ILC2比例,Spearman相关分析方法分析ILC2与上述疾病相关指标的相关性。将RA患者分为低疾病活动度(DAS28<3.2)和高疾病活动度(DAS28≥3.2)两组,流式细胞术检测白细胞介素13(IL-13)在RA患者ILC2的表达,并比较IL-13不同疾病活动度RA患者表达的差异。结果与健康人相比,RA患者外周血ILC2比例显著升高,ILC2绝对数增加,且与ESR、CRP、DAS28呈负相关。高疾病活动度RA患者ILC2分泌的IL-13显著低于低疾病活动度患者。结论ILC2通过分泌IL-13抑制RA患者疾病活动。Objective To study the changes of group 2 innate lymphoid cells(ILC2)in rheumatoid arthritis(RA)and its possible mechanism.Methods The 28 joint disease activity score(DAS28)and peripheral blood samples were collected from RA patients.Erythrocyto sedimentation rate(ESR)and C-reactive protein(CRP)were measured by an automatic erythrocyte sedimentation device and immune transmission turbidimetry,respectively.Rheumatoid factor(RF)and anti-cyclic citrullinated peptide antibody(ACCP)were assessed by immunoturbidimetry and chemiluminescence assay,respectively.Flow cytometry was used to detect the proportion of ILC2 in the patients with RA.The correlations between ILC2 and the above disease indexes were analyzed by Spearman correlation analysis.RA patients were divided into two groups:low disease activity(DAS28<3.2)group and high disease activity(DAS28≥3.2)group.The expression of interleukin-13(IL-13)in ILC2 of RA patients was detected by flow cytometry,and the differences of ILC2 expression were compared in RA patients with different disease activity of IL-13.Results Compared with healthy people,the proportion and absolute number of ILC2 in the peripheral blood of RA patients increased.ILC2 was negatively correlated with ESR,CRP and DAS28.IL-13 secreted by ILC2 in RA patients with high disease activity was significantly lower than that in the ones with low disease activity.Conclusion ILC2 inhibits RA disease activity by secreting IL-13.

关 键 词：类风湿性关节炎(RA) Ⅱ型固有淋巴样细胞(ILC2) 28个关节疾病活动度评分(DAS28) 白细胞介素13(IL-13) 

分 类 号：R593.22[医药卫生—内科学] R392.12[医药卫生—临床医学]