运动改善APP/PS1小鼠海马白质的机制研究  

Study on the Mechanism of Exercise Improving the White Matter of Hippocampus in APP/PS1 Mice

作　　者：闫清伟[1,2] 李亚楠 赵娜 徐亚涛[4] 李百侠 夏杰 徐波[3] YAN Qingwei;LI Yanan;ZHAO Na;XU Yatao;LI Baixia;XIA Jie;XU Bo(Xizang Minzu University,Xianyang 712082,China;Tibet Plateau Sports and Health Research Center,Xianyang 712082,China;East China Normal University,Shanghai 200241,China;Nanjing University of Finance&Economics,Nanjing 210046,China)

机构地区：[1]西藏民族大学,陕西咸阳712082 [2]西藏高原体育与健康研究中心,陕西咸阳712082 [3]华东师范大学,上海200241 [4]南京财经大学,江苏南京210046

出　　处：《中国体育科技》2020年第11期84-88,F0003,共6页China Sport Science and Technology

基　　金：西藏民族大学重大项目培育计划资助项目(19MDZ02);西藏高校教师专业实践实战能力提高计划项目;西藏自然科学基金重点项目。

摘　　要：目的:探讨运动对阿尔茨海默病小鼠海马白质的影响及机制。方法:将36只3月龄APP/PS1小鼠随机分为安静对照组(ADC,n=18)和运动干预组(ADE,n=18),对ADE组小鼠进行为期12周跑台运动干预。通过核磁共振弥散张量成像实验、免疫组织荧光实验、蛋白质印迹实验、正电子发射断层扫描/计算机断层扫描(positron emission tomography/computed tomography,PET/CT)实验对小鼠海马白质、髓鞘磷脂、葡萄糖代谢等相关指标进行检测。结果:与ADC组相比,ADE组小鼠发生以下变化,1)海马各向异性分数(fractional anisotropy,FA)值增高(P<0.05),MBP蛋白质表达量增高(P<0.01);2)磷脂代谢关键酶cPLA2蛋白质表达量降低(P<0.01),HADHA蛋白质表达量降低(P<0.01),SCOT蛋白质表达量降低(P<0.01);3)酮体转运载体MCT1、MCT2、MCT4蛋白质表达量均降低(P<0.05);4)海马葡萄糖相对摄取量增高(P<0.01),葡萄糖转运载体GLUT1、GLUT3蛋白质表达量均增高(P<0.01)。结论:12周跑台运动可抑制APP/PS1小鼠海马白质损伤,其机制可能与运动改善小鼠海马葡萄糖代谢,继而抑制髓鞘磷脂降解代谢有关。Objective:To explore the effect and mechanism of exercise on the white matter of hippocampus in Alzheimer’s disease.Methods:363-month-old APP/PS1 mice were randomly divided into two groups:quiet control group(ADC,n=18)and exercise intervention group(ADE,n=18).The related indexes of white matter,myelin and glucose metabolism in mouse hippocampus were detected by diffusion tensor imaging,immunofluorescence,Western blotting and PET/CT.Results:compared with ADC group,mice in ADE group had the following changes:1)increased FA value in hippocampus(P<0.05),increased MBP protein expression(P<0.01);2)decreased cPLA2 protein expression(P<0.01),decreased HADHA protein expression(P<0.01),decreased SCOT protein expression(P<0.01);3)decreased protein expression of ketone transporters MC1,MCT2 and MCT4(P<0.05);4)the relative uptake of glucose in hippocampus increased(P<0.01),and the expression of GLUT1 and GLUT3 protein in hippocampus increased(P<0.01).Conclusion:12 weeks treadmill exercise could inhibit the white matter damage in the hippocampus of APP/PS1 mice,and the mechanism might be related to the improvement of glucose metabolism in the hippocampus and then the inhibition of myelin degradation.

关 键 词：海马 白质 髓鞘碱性蛋白 葡萄糖转运体 能量代谢 

分 类 号：G804.2[文化科学—运动人体科学]