一株枯草芽孢杆菌对香蜂花的促生效果及关键代谢物积累的环境响应  

Prompting effects of an endophytic bacteria, Bacillus subtilis, on Melissa officinalis L. growth and response of its central secondary metabolic products to culturing conditions

作　　者：叶如梦 田锴 胡海静 万鹏伟 谭海霞 田兴军[1] 李朋富[1] YE Rumeng;TIAN Kai;HU Haijing;WAN Pengwei;TAN Haixia;TIAN Xingjun;LI Pengfu(School of Life Sciences,Nanjing University,Nanjing 210046,China;Key Laboratory of Ecological Security for Water Source Region of Mid-line Project of South-to-North Diversion Project of Henan Province,College of Agricultural Engineering,Nanyang Normal University,Nanyang 473061,China;Hebei University of Environment Engineering,Qinhuangdao 066102,China)

机构地区：[1]南京大学生命科学学院,南京210046 [2]南阳师范学院农业工程学院,河南省南水北调中线水源区生态安全重点实验室,南阳473061 [3]河北环境工程学院,秦皇岛066102

出　　处：《应用与环境生物学报》2020年第5期1035-1045,共11页Chinese Journal of Applied and Environmental Biology

基　　金：国家重点研发计划项目(2016YFD0600204);国家自然科学基金面上项目(31870598);国家自然科学基金重点项目(31530007);江苏省水利科技项目(ZQ2018107);河北科技厅重点研发专项(19273202D)资助。

摘　　要：内生菌对植物的促生作用在生产实践中得到了广泛运用.为探究植物内生菌对香料植物香蜂花(Melissa officinalisL.)生长和关键次生代谢物积累的影响及这种影响的栽培环境依赖性,利用一株内生枯草芽孢杆菌B117(Bacillus subtilis B117)灌根接种香蜂花,比较在不同土壤养分条件(较贫瘠组和营养组)下,B117对植株生长生理及总酚、总黄酮、迷迭香酸(RA)单位含量的影响.结果显示,B117对香蜂花有显著促生作用,接菌生长30 d后较贫瘠组和营养组株高分别增加了60.80%、25.50%,60 d后分别增加了45.81%和21.90%.B117对香蜂花的作用受土壤养分影响,较贫瘠组在接菌30 d和60 d后地上部干重增加了261.50%和9.55%,根长分别增加了49.92%和70.96%.接菌60 d后,较贫瘠组植株的超氧化物歧化酶(SOD)活性及营养组植株的过氧化物酶(POD)活性显著提升;营养组植株总酚、总黄酮、RA的单位含量及苯丙氨酸解氨酶(PAL)活性显著低于较贫瘠组.接菌显著降低了营养组单位含量的总酚和RA,但对较贫瘠组无显著影响,即B117在增加植株生物量的基础上,并未显著降低较贫瘠组关键次生代谢物的积累.综上所述,内生菌B117对香蜂花的作用依赖土壤营养条件,在适当贫瘠的土壤中接种B117既能增加香蜂花植株的生物量又能保障其核心代谢产物的产量.(图4表3参55附图6)Endophytic bacteria are widely used in plant cultivation. In this study, a potted experiment was conducted to investigate the influences of an endophytic bacteria strain, Bacillus subtilis B117, on the growth and secondary metabolite accumulation in an aromatic herb, Melissa officinalis L. B117 was administered to M. officinalis through an injured root-irrigation method. Plants were cultured in two contrasting conditions(oligotrophic soil and fertilized soil). Treatment effects were explored by investigating the variations in plant growth, physiological reactions, as well as the contents of secondary metabolites that were represented by total phenolic content, total flavonoids, and rosemary acid(RA). The inoculation of B117 had significant effects on plant growth, increasing the plant height in oligotrophic and fertilized soil by 60.80% and 25.50%, respectively, after 30 days and by 45.81% and 21.90%, respectively, after 60 days of inoculation. The effects of B117 on plants was influenced by soil nutrient conditions. In the oligotrophic environment, B117 increased the dry weight of aerial parts by 261.50% and 9.55% and increased the root length by 49.92% and 70.96% after 30 and 60 days inoculation, respectively. After 60 days, B117 significantly increased the activity of SOD in oligotrophic soil and the POD activity in fertilized soil. The total phenolic content, total flavonoids, phenylalanine ammonia-lyase(PAL) activity, and RA in plants were higher in the oligotrophic soil than in the fertilized environment. B117 significantly reduced the total phenolic content and RA in the fertilized group, whereas there was no effect observed in the oligotrophic environment. Consequently, B117 had profound growth-promotion effects on M. officinalis and did not reduce the accumulation of central secondary metabolites in the oligotrophic soil. In conclusion, the effect of B117 on M. officinalis depends on soil nutrient conditions. Inoculating B117 in moderately barren soil would increase the yield of M. officinalis biomass as

关 键 词：促生作用 迷迭香酸 土壤养分 香蜂花 植物内生菌 

分 类 号：S567.239[农业科学—中草药栽培]