尖海龙共附生真菌Talaromyces amestolkiae的化学成分  

Chemical constituents of Talaromyces amestolkiae, a symbiotic fungus of Syngnathus acus

作　　者：金梦莹 陈金凤 曹钰镁 沈芋蓉 李新爱 侯胜宏 郭大乐[1] 罗禹 邓赟[1] JIN Mengying;CHEN Jinfeng;CAO Yumei;SHEN Yurong;LI Xinai;HOU Shenghong;GUO Dale;LUO Yu;DENG Yun(Key Laboratory of Standardization of Chinese Herbal Medicine of Ministry of Education/Sichuan Provincial Key Laboratory of Systematic Research,Development and Utilization of Chinese Medical Resources:Ministry of State Key Laboratory Breeding Base/School of Pharmacy,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China;School of Pharmacy,Southwest Medical University,Luzhou 646000,China;Sichuan Heyi Pharmaceutical Co.,Ltd,Chengdu 611630,China)

机构地区：[1]成都中医药大学药学院中药材标准化教育部重点实验室,四川省中药资源系统研究与开发利用重点实验-省部共建国家重点实验室培育基地,成都611137 [2]西南医科大学药学院,泸州646000 [3]四川禾亿制药有限公司,成都611630

出　　处：《应用与环境生物学报》2020年第5期1230-1234,共5页Chinese Journal of Applied and Environmental Biology

基　　金：国家自然科学基金面上项目(81373961);四川省科技厅省青年科技创新研究团队专项计划(2016TD0006);中国博士后科学基金(2017M622985);四川省教育厅科研项目(18ZA0191);成都中医药大学青年教师创新基金(ZRQN1766)资助。

摘　　要：对尖海龙共附生真菌Talaromyces amestolkiae的化学成分进行研究.采用固体培养基对该菌株进行培养,利用硅胶柱、Sephdex-LH-20凝胶柱、MCI柱、制备色谱等方法对化学成分进行分离纯化,并运用质谱、核磁共振等方法进行结构鉴定.共分离得到10个化合物,分别为methyl 2-formyl-4-methylfuro[3,2-b]pyrrole-5-carboxylate(1)、4-甲基苔黑酚(2)、麦角甾-5,7,22-三烯-3-醇(3)、3-甲氧基苯酚(4)、thailandolide B(5)、aspergillumarin B(6)、(R)-3-(3-hydroxypropyl)-8-hydroxy-3,4-dihydroisocoumarin(7)3-hydroxymethyl-6,8-dimethoxycoumarin(8)、penicifuran D(9)、penicifuran C(10).其中化合物1为首次分离得到的天然产物,化合物2-10均首次从T.amestolkiae中分离.(参33)This study focused on the chemical constituents of Talaromyces amestolkiae, a symbiotic fungus obtained from Syngnathus acus Linnaeus. Talaromyces amestolkiae was cultured with solid-state fermentation, and its secondary metabolites were separated by silica gel, Sephadex LH-20, MCI column, as well as preparative chromatography. The subsequent compounds were identified by spectral methods. Ten compounds were isolated and identified as methyl 2-formyl-4-methylfuro[3,2-b]pyrrole-5-carboxylate(1), 4-methylorcnol(2), erg osta-5,7,22-trien-3-ol(3),3-methoxyphenol(4), thailandolide B(5), aspergillumarin B(6),(R)-3-(3-hydroxypropyl)-8-hydroxy-3,4-dihydroisocoumarin(7), 3-hydroxymethyl-6,8-dimethoxycoumarin(8), penicifuran D(9), penicifuran C(10). Compound 1 was obtained from natural source for the first time. Compounds 2-10 were isolated from this genus for the first time.

关 键 词：尖海龙 共附生真菌 Talaromyces amestolkiae 化学成分 

分 类 号：R284.1[医药卫生—中药学]