云南石屏汉族与彝族患者外阴阴道念珠菌病的菌种构成  

Study on the strain composition of vulvovaginal candidiasis of Han and Yi ethnic in Shiping County,Yunnan Province

作　　者：高飞[1] 郑永钦[2] 曹萍[1] 贺军栋[2] 杨稳莉 梁舒雯 姚岚[5] 撒亚莲[2] GAO Fei;ZHEGN Yong-qin;CAO Ping(Department of Dermatology,The First People’s Hospital of Yunnan Province,Kunming650032,China)

机构地区：[1]云南省第一人民医院(昆明理工大学附属医院)皮肤科,云南昆明650032 [2]云南省第一人民医院(昆明理工大学附属医院)临床基础研究所,云南昆明650032 [3]云南省第一人民医院(昆明理工大学附属医院)核医学科,云南昆明650032 [4]石屏县人民医院妇科 [5]昆明市中医医院针灸科

出　　处：《中国实验诊断学》2020年第10期1590-1594,共5页Chinese Journal of Laboratory Diagnosis

基　　金：云南省卫健委内设机构资助项目(2017NS228)。

摘　　要：目的探讨云南省石屏县汉族和彝族患者外阴阴道念珠菌病(VVC)的菌种构成情况。方法收集来源于云南省石屏地区念珠菌病患者的阴道分泌物143份,其中汉族84份,彝族59份;根据科玛嘉(CHROMagar)显色培养基人工鉴定结果,同时经PCR扩增ITS1-ITS4区段,用测序法进行菌种鉴定,用DNAStar软件对测序结果进行分析。将科玛嘉显色培养基法与测序结果进行对比。结果科玛嘉显色培养基法检出4种菌种,分别是光滑念珠菌,克柔念珠菌,白色念珠菌和热带念珠菌。测序法在VVC阴道分泌物中检测出5种菌种,分别是白色念珠菌113株、光滑念珠菌25株、近平滑念珠菌3株、热带念珠菌和季也蒙念珠菌各1株;汉族患者未检测到季也蒙念珠菌,彝族患者未检测到热带念珠菌。汉族患者的白色念珠菌、光滑念珠菌、近平滑念珠菌和热带念珠菌的携带率分别为78.57%(66株)、17.86%(15株)、2.38%(2株)和1.19%(1株);彝族患者的白色念珠菌、光滑念珠菌、近平滑念珠菌和季也蒙念珠菌的携带率分别为79.66%(47株)、16.95%(10株)、1.69%(1株)和1.69%(1株)。云南省石屏县汉族VVC患者的念珠菌菌种构成比与彝族患者相比较,差异无统计学意义(P>0.05)。结论PCR测序法优于科玛嘉显色培养基法鉴定念珠菌的菌种,云南省石屏县汉族与彝族VVC患者的病原菌以白色念珠菌为主。Objective To investigate the composition of strains from vulvovaginal candidiasis(VVC)patients within Han ethnic and Yi ethnic living in Shiping county of Yunnan Province.Methods 143vaginal secretions from candidiasis patients in Shiping,Yunnan Province,84from Han and 59from Yi were collected.Identification results came out according to the CHROMagar.Meanwhile the ITS1-ITS4region was amplified by PCR,and the strains were identified by sequencing method.The results were analyzed by DNA Star software.The results of CHROMagar and sequencing were compared.Results Four strains of Candida,including C.albicans,C.glabrata,C.parasilosis,C.tropicalis were detected by CHROMagar method.Five strains of Candida were detected by sequencing in vaginal secretions of VVC.There were strains 113strains identified as C.albicans,25strains as C.glabrata,3strains as C.parasilosis,1strains as C.guilliermondii,and 1strain as C.tropicalis.C.guilliermondii was not observed in Han ethnic,whereas C.tropicalis didn’t appeared in Yi ethnic.The composition ratio was composed by C.albicans,C.glabrata,C.parasilosis,C.tropicalis,and C.guilliermondii in Han ethnic,was 78.57%(66),17.86%(15),2.38%(2),1.19%(1)and 0.00%(0)respectively.The composition ratio in Yi ethnic was 79.66%(47),16.95%(10),1.69%(1),0.00%(0)and 1.69%(1),respectively.No statistical difference in the composition ratio of Candida strains was observed between the Han ethnic and Yi ethnic(P>0.05).Conclusion The results showed that the PCR sequencing method was superior to the CHROMagar method in identifying Candida species.C.albicans was the main pathogen of VVC patients of Han and Yi ethnic in Shiping County,Yunnan Province.

关 键 词：外阴阴道念珠菌病 内含子转录间隔区 汉族 彝族 菌种构成 

分 类 号：R756.5[医药卫生—皮肤病学与性病学]