预防2号方合剂的质量标准研究  

Study on the Quality Standards of No.2 Prescription Mixture(预防2号方合剂)

作　　者：黄涛 欧阳波[1] 肖作奇 潘涛[1] 黄懿[1] 邱盼子 HUANG Tao;OUYANG Bo;XIAO Zuo-qi;PAN Tao;HUANG Yi;QIU Pan-zi(Hunan Provincial Maternal and Child Health Care Hospital,Changsha Hunan 410008,China)

机构地区：[1]湖南省妇幼保健院,湖南长沙410008

出　　处：《中医药导报》2020年第15期14-16,28,共4页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：湖南省自然科学基金(2019JJ80103);湖南省中医药管理局一般项目(2019106)。

摘　　要：目的:建立预防2号方合剂的质量标准。方法:采用薄层色谱法对处方中黄芪、山银花和陈皮进行定性鉴别;采用HPLC-DAD法测定绿原酸、橙皮苷、川续断皂苷乙的含量。结果:定性鉴别分离度好,专属性强;绿原酸、橙皮苷、川续断皂苷乙的质量浓度分别在24.69~185.16μg/mL、2.08~62.40μg/mL和18.57~185.66μg/mL内呈良好的线性关系(r值均≥0.999,7),3种有效成分方法回收率均符合含量测定要求。结论:该方法简单准确,重复性好,可为预防2号方合剂的质量控制提供参考。Objective:To establish a quality standard for No.2 Prescription Mixture.Methods:Thin-layer chromatography(TLC)was used to qualitatively identify Huangqi(Astragalus),Shanyinhua(Lonicerae flos)and Chenpi(Tangerine peel)in the prescription.HPLC-DAD method was used to determine the content of chlorogenic acid,hesperidin,and dipsacoside B.Results:The qualitative identification has good resolution and strong specificity;the mass concentrations of chlorogenic acid,hesperidin,and dipsacoside B showed a good linear relationship in the range of 24.69-185.16,2.08-62.40,18.57-185.66μg/mL,respectively(r≥0.999,7),The recovery rates of the three active ingredient all met the content determination requirements.Conclusion:The method is simple,accurate,and reproducible.It can provide a reference for the quality control of No.2 Prescription Mixture.

关 键 词：预防2号方 质量标准 黄芪甲苷 绿原酸 橙皮苷 川续断皂苷乙 

分 类 号：R284.1[医药卫生—中药学]