复方黄连灌肠液体外抑菌活性的研究  

Study on Antibacterial Activity of Fufang Huanglian Guanchangye(复方黄连灌肠液)in Vitro

作　　者：倪晓霞[1] 叶财发 沈秋莲 陈锦珊[1] NI Xiao-xia;YE Cai-fa;SHEN Qiu-lian;CHEN Jin-shan(The 909th Hospital of Joint Logistics Support Force of Peopled Liberation Army/The Affiliated Dongnan Hospital of Xiamen University,Zhangzhou Fujian 363000,China)

机构地区：[1]中国人民解放军联勤保障部队第909医院/厦门大学附属东南医院,福建漳州363000

出　　处：《中医药导报》2020年第15期25-28,共4页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：医院青年苗圃课题项目(16Y025)。

摘　　要：目的:评价复方黄连灌肠液的体外抑菌活性。方法:采用倍比稀释法考察复方黄连灌肠液对大肠埃希菌、沙门氏菌、双歧杆菌、白色念珠菌等8种代表性菌株的体外抑菌活性,测定抑菌圈大小、最小抑菌浓度(MIC)和最低杀菌浓度(MBC)。结果:复方黄连灌肠液对8种代表性菌株均呈现不同程度的抑菌作用,以金黄色葡萄球菌最敏感;金黄色葡萄球菌的MIC为0.027,5 mg/mL,沙门氏菌、铜绿假单胞菌的MIC为0.055,0 mg/mL,大肠埃希菌、枯草芽孢杆菌、双歧杆菌、白色念珠菌、黑曲霉菌的MIC为0.110,0mg/mL;对金黄色葡萄球菌、沙门氏菌的MBC为0.055,0 mg/mL,铜绿假单胞菌、枯草芽孢杆菌、双歧杆菌、白色念珠菌的MBC为0.110,0 mg/mL;大肠埃希菌、黑曲霉菌的MBC为0.220,0 mg/mL;复方黄连灌肠液对8种代表性菌种的抑菌圈均在10~20 mm范围内,属于中度敏感。结论:复方黄连灌肠液具有明显的体外抑菌活性,对肠道常见菌群具有广谱的抑菌作用。Objective:To evaluate the antibacterial activity of Fufang Huanglian Guanchangye in vitro.Methods:The method of multiple dilution was used to investigate the in vitro antibacterial activity of Fufang Huanglian Guanchangye against 8 representative strains of Escherichia coli,Salmonella,Bifidobacterium,Candida albicans and so on.The size of inhibition zone,minimum inhibitory concentration(MIC)and minimum bactericidal concentration(MBC)were determined.Results:Fufang Huanglian Guanchangye showed varying degrees of antibacterial effect on 8 representative strains,with Staphylococcus aureus being the most sensitive.The MIC of Staphylococcus aureus was 0.027,5 mg/mL,the MIC of Salmonella and Pseudomonas aeruginosa was 0.055,0 mg/mL,the MIC of Escherichia coli,Bacillus subtilis,Bifidobacterium,Candida albicans,and Aspergillus niger was0.110,0 mg/mL,the MBC of Staphylococcus aureus and Salmonella was 0.055,0 mg/mL,the MBC of Pseudomonas aeruginosa,Bacillus subtilis,Bifidobacterium,and Candida albicans was 0.110,0 mg/mL,the MBC of Escherichia coli and Aspergillus niger was 0.220,0 mg/mL.The inhibition zone of Fufang Huanglian Guanchangye to the 8 representative bacterial species was within the range of 10-20 mm,which is moderately sensit ive.Conclusion:Fufang Huanglian Guanchangye has obvious antibacterial activity in vitro and broad-spectrum antibacterial effect on common intestinal flora.

关 键 词：复方黄连灌肠液 体外抑菌活性 抑菌圈 最小抑菌浓度 最低杀菌浓度 

分 类 号：R285.5[医药卫生—中药学]