Chiralcel OD-H手性柱拆分醇类对映体  

Enantioseparation of Alcohols by Chiralcel OD-H Column

作　　者：朱濛恩 张诗楠 李国祥 ZHU Mengen;ZHANG Shinan;LI Guoxiang(School of Chemical and Environmental Engineering,Hubei Minzu University,Enshi 445000,China;Hubei Key Laboratory of Biological Resources Protection and Utilization(Hubei Minzu University),Enshi 445000,China)

机构地区：[1]湖北民族大学化学与环境工程学院,湖北恩施445000 [2]生物资源保护与利用湖北省重点实验室(湖北民族大学),湖北恩施445000

出　　处：《湖北民族大学学报（自然科学版）》2020年第4期367-370,381,共5页Journal of Hubei Minzu University:Natural Science Edition

基　　金：国家自然科学基金项目(51563007);生物资源保护与利用湖北省重点实验室开放基金项目(PT012004).

摘　　要：以Chiralcel OD-H手性商品柱在正相条件下对9种醇类对映异构体进行拆分,考察了流速、流动相中异丙醇含量、柱温等主要因素对手性拆分的影响.试验结果表明:当流动相为正己烷∶异丙醇(90∶10,v/v)、流速为0.5 mL/min、柱温为25℃、检测波长为254 nm时,8种手性醇得到分离,其中7种化合物达到基线分离,且分离度分别为R s1=12.73、R s 3=4.67、R s4=1.75、R s5=11.80、R s6=1.84、R s8=2.22、R s9=12.34,对映体在色谱固定相上的分离过程中主要受吸附焓控制.该方法快速、高效、简便,适用于手性醇类化合物的拆分.Nine alcohol enantiomers were efficiently separated by Chiralcel OD-H column under normal phase conditions.The main factors such as flow rate,isopropanol content in mobile phase and column temperature on chiral resolution were investigated respectively.The results indicated that eight chiral alcohols were separated under the given chromatographic conditions,and seven compounds of them achieved baseline separation.When n-hexane:isopropanol(90∶10,v/v)was used as mobile phase,flow rate was 0.5 mL/min,column temperature was 25℃,and detection wavelength was 254 nm,the resolution was R s1=12.73、R s3=4.67、R s4=1.75、R s5=11.80、R s6=1.84、R s8=2.22、R s9=12.34,respectively.Enantioseparation of alcohols on chromatographic stationary phase was mainly controlled by adsorption enthalpy.The proposed method is fast,efficient and simple,and suitable for the resolution of chiral alcohols.

关 键 词：高效液相色谱 Chiralcel OD-H色谱柱 手性醇 对映体拆分 外消旋体 

分 类 号：O636.9[理学—高分子化学]