碳氮比对SBR系统硝化过程及EPS三维荧光光谱特性的影响  

Effect of C/N ratio on the performance of nitrification and three-dimensional fluorescence excitation-emission matrix(EEM)spectroscopy characterization of extracellular polymeric substances from sequencing batch reactors

作　　者：陈翠忠 李俊峰[1] 蓝明菊[1] 额热艾汗 谢可飞 徐威龙 刘雅茹 孙洪伟 CHEN Cuizhong;LI Junfeng;LAN Mingju;EREAIHAN;XIE Kefei;XU Weilong;LIU Yaru;SUN Hongwei(College of Water Conservancy and Architectural Engineering,Shihezi University,Shihezi 832000,Xinjiang,China;School of Environmental and Material Engineering,Yantai University,Yantai 264005,Shandong,China)

机构地区：[1]石河子大学水利建筑工程学院,新疆石河子832000 [2]烟台大学环境与材料工程学院,山东烟台264005

出　　处：《化工进展》2020年第12期5275-5282,共8页Chemical Industry and Engineering Progress

基　　金：国家自然科学基金重点项目-新疆联合基金(U1803244);国家重点研发计划(2017YFC0404304);兵团重点领域科技攻关计划(2019AB035);石河子大学科研项目(ZZZC201905B)。

摘　　要：以人工模拟废水为研究对象,采用4组间歇式活性污泥(SBR)反应器(R0、R5、R(10)和R(15)),探究了硝化过程中碳氮比(C/N)对NH4+-N去除和胞外聚合物(EPS)含量的影响。同时采用三维荧光光谱技术(3D-EEM)结合积分区域法(FRI),分析进出水时EPS各组分所占比例及变化情况。结果表明,4种C/N系统均获得了较高的NH4+-N去除率,且氨氧化速率与C/N呈负相关;C/N升高(0→15),进出水时的EPS及其组分含量随之增大,其中C/N对PS含量影响尤为显著,且硝化过程中多糖(PS)积累。根据荧光区域积分法(FRI)分析,不同C/N条件下EPS各荧光区域强度百分比存在显著差异,其中荧光区域V(腐殖酸类物质,46%~57%)的强度百分比值(P(i,n))最高。此外,蛋白质类物质酪氨酸和色氨酸以及溶解性微生物代谢产物在硝化过程中均被微生物所利用。This study focused on the effect of C/N ratio on the removal efficiency of NH+4-N and extracellular polymeric substance(EPS)during nitration process by using the sequencing batch reactor(SBR)to treat synthetic wastewater under four C/N ratio conditions(0,5,10,15).Three-dimensional fluorescence spectroscopy(3D-EEM)coupled with fluorescence regional integration(FRI)was used to analyze the constituents of EPS.The results showed that the high removal efficiency of NH+4-N was obtained and the ammoxidation rate was negatively correlated with the C/N ratio.When the C/N ratio increased from 0 to 15,the content of influent and effluent EPS and its components increased,the effect of C/N ratio on PS was significant,and the content of PS was accumulated during nitrification.According to fluorescence region integral(FRI)analysis,there was remarkable difference in the fluorescence response percentage of EPS for different C/N ratio,and region V(humic acid substance,46%—57%)was the dominant fraction in EPS under different C/N conditions.In addition,the protein-like substance tyrosine and tryptophan,and soluble microbial by-product were utilized during nitrification.

关 键 词：硝化污泥 碳氮比 氨氧化 胞外聚合物 三维荧光光谱 荧光区域积分 

分 类 号：X52[环境科学与工程—环境工程]