黄褐毛忍冬对大鼠急性肺损伤模型的抗炎机制研究  

Study on anti-inflammatory mechanism of Lonicera fulvotometosa on acute lung injury model in rats

作　　者：周枝 刘畅 俸婷婷 张敏 高月 周英 ZHOU Zhi;LIU Chang;FENG Ting-ting;ZHANG Min;GAO Yue;ZHOU Ying(School of Liquor and Food Engineering,Guizhou University,Guiyang 550025,China;School of Pharmacy,Guizhou University of Traditional Chinese Medicine,Guiyang 550025,China;National&Local Joint Engineering Research Center for the Exploitation and Utilization Technology of Medicine and Food Dual-use Resources in the Southwest of China,Guiyang 550025,China)

机构地区：[1]贵州大学酿酒与食品工程学院,贵州贵阳550025 [2]贵州中医药大学药学院,贵州贵阳550025 [3]西南药食两用资源开发利用技术国家地方联合工程研究中心,贵州贵阳550025

出　　处：《中国中药杂志》2020年第20期4978-4983,共6页China Journal of Chinese Materia Medica

基　　金：贵州省科技攻关计划项目(黔科合[2016]支撑2909);贵州省高层次创新型人才培养项目(黔科合人才[2015]4032号)。

摘　　要：研究黄褐毛忍冬对脂多糖(LPS)所致急性肺损伤(ALI)大鼠肺组织炎症通路中相关基因的表达,初步探讨该提取物的护肺作用及炎症机制,为黄褐毛忍冬在临床抗炎应用提供实验和理论依据。将40只SD雄性大鼠随机分为4组:正常组、模型组、黄褐毛组(7.2 g·kg-1)和阳性药组(地塞米松,5 mg·kg-1)。黄褐毛组每天灌胃给药1次,连用5 d。阳性药组于造模前2 h一次性腹腔注射地塞米松;除正常组外,其余组均予腹腔注射脂多糖(5 mg·kg-1)诱导ALI模型。造模6 h后取血、肺泡灌洗液(BALF)和肺组织。对肺组织进行病理学观察分析,采用酶联免疫吸附(ELISA)法检测血清、BALF及肺组织中炎症因子的含量,通过实时荧光定量聚合酶链式反应(qRT-PCR)法检测肺组织中白细胞介素(IL)1R1、IL6R、肿瘤坏死因子-α(TNF-α)诱导蛋白3(TNFAIP3)和核因子κB抑制剂α(NFKBIA)的表达。黄褐毛组较模型组肺损伤程度轻微;与模型组相比,黄褐毛组和地塞米松组显著降低了ALI大鼠血清和BALF的白细胞介素-6(IL-6)、白细胞介素1β(IL-1β)、TNF-α及肺组织的丙二醛(MDA)、髓过氧化物酶(MPO)浓度,明显升高肺组织中谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)的浓度;黄褐毛组肺组织TNFAIP3,IL1R1,IL6R和NFKBIA mRNA的表达较模型组显著降低。黄褐毛忍冬水提物预先给药,可降低急性肺损伤大鼠炎症因子的含量,下调肺组织相关基因的表达,具有显著的抗炎效果;其机制可能与调控转录因子(NF-κB)信号通路有关,通过下调下游相关炎症因子的表达,减轻肺部炎症反应。To study the effect of Lonicera fulvotometosa(LFH) on expression of genes related to inflammatory pathways in the lung tissue of rats with acute lung injury(ALI) induced by lipopolysaccharide(LPS), explore the lung-protective effects and inflammatory mechanisms of L. fulvotometosa water extract, and provide experimental and theoretical basis for the clinical application of LFH. Forty SD rats were randomly divided into 4 groups: normal group, model group(LPS, 5 mg·kg-1), LFH group(7.2 g·kg-1) and dexa-methasone group(Dexa, 5 mg·kg-1). The rats in LFH group received intragastric administration of water extract once a day for 5 days;rats in dexamethasone group received intraperitoneal injection for 2 hours before modeling. Except the normal group, the rats in other groups were injected intraperitoneally with LPS(5 mg·kg-1) to induce ALI rats model. Serum, bronchoalveolar lavage fluid(BALF) and lung tissues were collected 6 hours after modeling. The lung tissues were taken for pathological observation;enzyme-linked immunosorbent assay(ELISA) was used to detect changes of inflammatory factors in serum and BALF;Real-time quantitative polymerase chain reaction(RT-qPCR) was applied to detect mRNA expression of tumor necrosis factor alpha inducible protein 3(TNFAIP3), interleukin(IL) 1 R1, interleukin(IL) 6 R and nuclear factor κB inhibitor α(NFKBIA) in the lung tissues. The degree of lung injury was lighter in LFH group than that in the LPS group. As compared with the LPS group, the levels of interleukin-1β(IL-1β), interleukin-6(IL-6), tumor necrosis factor-α(TNF-α) in serum and BALF, malondialdehyde(MDA) and myeloperoxidase(MPO) in lung tissues were significantly reduced in LFH group and Dexa group, while glutathione peroxidase(GSH-Px), superoxide dismutase(SOD) in lung tissues were significantly increased;the mRNA expression of TNFAIP3, IL1 R1, IL6 R and NFKBIA in the lung tissues of the LFH group was significantly lower than that of the LPS group. The water extract of LFH can significantly reduce the content

关 键 词：黄褐毛忍冬 脂多糖 急性肺损伤 炎症通路 NF-ΚB 

分 类 号：R285.5[医药卫生—中药学]