不同营养盐及浓度对黄丝藻Tribonema sp.FACHB-1786生长及脂质积累的影响  

Effects of substrate on growth and lipid accumulation of Tribonema sp.FACHB-1786

作　　者：张婷[1,5] 何青 徐梓钧 索菲娅 张成武[3] 胡强[2,4] Ting Zhang;Qing He;Zijun Xu;Feiya Suo;Chengwu Zhang;Qiang Hu(College of Life Science and Technology,Xinjiang University,Urumchi 830046,Xinjiang,China;Center for Microalgal Biotechnology and Biofuels,Institute of Hydrobiology,Chinese Academy of Sciences,Wuhan 430072,Hubei,China;Research Center of Hydrobiology,Department of Ecology,Jinan University,Guangzhou 510632,Guangdong,China;Microalgal Biotechnology Center,SDIC Biotech Investment Corporation,Beijing 100142,China;Urumqi Vocational University,Urumchi 830012,Xinjiang,China)

机构地区：[1]新疆大学生命科学技术学院,新疆乌鲁木齐830046 [2]中国科学院水生生物研究所,湖北武汉430072 [3]暨南大学水生生物研究中心,广东广州510632 [4]国投生物科技投资公司,北京100142 [5]乌鲁木齐职业大学,新疆乌鲁木齐830012

出　　处：《生物工程学报》2020年第11期2478-2493,共16页Chinese Journal of Biotechnology

基　　金：国家开发投资有限公司项目(No.SDICMBC-00003)资助。

摘　　要：丝状微藻黄丝藻Tribonema sp.具有抗浮游动物捕食、易收获、油脂含量高等优点,且其脂肪酸组分中含有丰富的棕榈油酸(Palmitoleic acid,PA)和二十碳五烯酸(Eicosapentaenoic acid,EPA),被认为是生产生物柴油和高附加值产品的重要原料。为了提高黄丝藻脂质生产效率,文中研究了不同浓度的氮(NaNO3:255–3060 mg/L)、磷(K2HPO4:4–240 mg/L)、铁((NH4)3FeC12H10O14:0.6–12 mg/L)、镁(MgSO4:7.5–450 mg/L)元素对黄丝藻FACHB-1786生长、油脂积累和脂肪酸组分的影响。结果表明,培养基中磷、铁、镁三种元素的浓度对黄丝藻生长具有显著影响,其中增加MgSO4浓度可显著提高黄丝藻的生物量,当MgSO4浓度增加至450 mg/L时,获得最大生物量为8.09 g/L,显著高于目前报道的有关黄丝藻自养条件下获得的生物量;氮元素浓度对黄丝藻的生长没有显著影响(P>0.05),但高浓度氮元素有利于黄丝藻脂质的积累;黄丝藻FACHB-1786在765 mg/L NaNO3、80 mg/L K2HPO4、6 mg/L(NH4)3FeC12H10O14、75 mg/L MgSO4的营养盐条件下可获得最大总脂单位体积产率、棕榈油酸和EPA产率,分别为319.6 mg/(L·d)、135.7 mg/(L·d)和24.2 mg/(L·d)。研究结果为后期黄丝藻的生产应用提供一定的理论依据和参考。Filamentous microalga Tribonema sp.has the advantages of highly resistance to zooplankton-predation,easy harvesting,and high cellular lipid content,in particular large amounts of palmitoleic acid(PA)and eicosapentaenoic acid(EPA).Therefore,Tribonema sp.is considered as a promising biomass feedstock to produce biodiesel and high-value products.In this work,we studied the effect of different concentrations of nitrogen(NaNO3:255–3060 mg/L),phosphorus(K2HPO4:4–240 mg/L),iron((NH4)3FeC12H10O14:0.6–12 mg/L)and magnesium(MgSO4:7.5–450 mg/L)on the biomass,lipid content,and fatty acid composition of Tribonema sp.FACHB-1786,aiming at enhancing cell lipid productivity.The growth of Tribonema sp.had a positive correlation with the concentration of magnesium,and the maximum biomass of Tribonema sp.(under the condition of 450 mg/L MgSO4)was 8.09 g/L,much greater than those reported in previous studies using the same and other Tribonema species under autotrophic conditions.Different nitrogen concentrations exerted no significant effect on algal growth(P>0.05),but a higher nitrogen concentration resulted in a greater amount of lipid in the cells.The maximum volumetric productivities of total lipids(319.6 mg/(L·d)),palmitoleic acid(135.7 mg/(L·d)),and eicosapentaenoic acid(24.2 mg/(L·d))of Tribonema sp.were obtained when the concentrations of NaNO3,K2HPO4,(NH4)3FeC12H10O14,and MgSO4 were 765 mg/L,80 mg/L,6 mg/L,and 75 mg/L,respectively.This study will provide a reference for substrate optimization for Tribonema sp.growth and lipid production.

关 键 词：黄丝藻 矿质元素 藻油 棕榈油酸 二十碳五烯酸 微藻培养 

分 类 号：X52[环境科学与工程—环境工程]