三七总黄酮抗糖皮质激素诱发骨质疏松的效应  

Antagonistic Effect of Total Flavonoids of Panax notoginseng on Osteoporosis Induced by Glucocorticoid

作　　者：吴丽园 罗夙医 顾永洁 王旗鹏 许家豪 WU Li-yuan;LUO Su-yi;GU Yong-jie;WANG Qi-peng;XU Jia-hao(Basic Medical College of Kunming Medical University,Kunming Yunnan 650500,China)

机构地区：[1]昆明医科大学基础医学院,云南昆明650500

出　　处：《昆明医科大学学报》2020年第11期25-29,共5页Journal of Kunming Medical University

基　　金：云南省教育厅科学研究基金资助重点项目(2015Z075)。

摘　　要：目的研究三七总黄酮拮抗糖皮质激素诱发骨质疏松的效应及可能的机制。方法将50只体重约170 g的SPF级雌性SD大鼠随机分为五组(10只/组),即正常对照组、模型组(激素组)和三七总黄酮高、中、低剂量治疗组(激素+用药组)。模型组和三七总黄酮治疗组采用地塞米松(2.5 mg/kg)肌肉注射,每周2次;同时三个治疗组分别给予三七总黄酮275 mg/(kg·d)、137.5 mg/(kg·d)、68.75 mg/(kg·d)灌胃治疗,每天1次。实验期9周。9周后测定各组大鼠股骨骨密度、反映骨生物力学性能的指标及血清生化指标。结果与正常组比较,模型组大鼠股骨骨密度(BMD)、骨生物力学性能、血清钙水平明显降低,血清磷水平及血清碱性磷酸酶活性明显升高(P<0.05);与模型组比较,三七总黄酮高、中剂量治疗组大鼠股骨BMD、骨生物力学性能、血清钙水平明显增高,血清磷水平及血清碱性磷酸酶活性明显降低(P<0.05)。结论三七总黄酮可提高糖皮质激素性骨质疏松大鼠骨密度,改善其骨生物力学性能,对糖皮质激素诱发的骨质疏松具有拮抗作用,作用机制与其抑制骨吸收及糖皮质激素诱导的钙磷代谢失衡有关。Objective To study the antagonistic effect of total flavonoids of Panax notoginseng on osteoporosis induced by glucocorticoid and its possible mechanism.Methods Fifty female SD rats(SPF-grade)weighing about 170 g were randomly divided into five groups(10/group):normal control group,model group(hormone group)and high,medium and low dose treatment group(hormone+drug group)of total flavonoids of Panax notoginseng.Dexamethasone(2.5 mg/kg)was injected intramuscularly twice a week in the model group and total flavonoids of Panax notoginseng treatment groups.At the same time,the three treatment groups were given total flavonoids of Panax notoginseng 275 mg/(kg·d),137.5 mg/(kg·d)and 68.75 mg/(kg·d)by intragastric administration once a day.The experimental period was 9 weeks.After 9 weeks,bone mineral density(BMD)and indexes reflecting biomechanical properties of femur,serum biochemical indexes were measured.Results Compared with the normal group,the bone mineral density of femur,bone biomechanical properties and the serum calcium level decreased significantly,the serum phosphorus level and the serum alkaline phosphatase activity increased significantly in the model group(P<0.05).Compared with the model group,the bone mineral density,bone biomechanical properties and the serum calcium level increased significantly,the serum phosphorus level and the serum alkaline phosphatase activity decreased significantly in the high and medium dose treatment group of total flavonoids of Panax notoginseng(P<0.05).Conclusions Total flavonoids of Panax notoginseng can increase the bone density and improve the biomechanical properties of bone in glucocorticoid-induced osteoporosis rats.It has an antagonistic effect on osteoporosis induced by glucocorticoid.The mechanism of action is related to the inhibition of bone absorption and the imbalance of calcium and phosphorus metabolism induced by glucocorticoid.

关 键 词：三七总黄酮 糖皮质激素 骨质疏松症 骨密度 骨生物力学性能 

分 类 号：R285.5[医药卫生—中药学]