microRNA-146a与冠心病患者斑块稳定性的相关性  

Relationship between microRNA-146a and the Plaque Stability in Coronary Heart Disease Patients

作　　者：徐栩[1] 武力勇[2] 周明礼[2] 朱国富[2] XU Xu;WU Li-yong;ZHOU Ming-li;ZHU Guo-fu(Dept,of Geriatrics,Ganmei Hospital,Kunming First People's Hospital,Kunming Yunnan 650011;Dept,of Cardiology,The 2nd Affiliated Hospital of Kunming Medical University,Kunming Yunnan 650101,China)

机构地区：[1]昆明市第一人民医院甘美医院老年病科,云南昆明650011 [2]昆明医科大学第二附属医院心血管内科,云南昆明650101

出　　处：《昆明医科大学学报》2020年第11期56-61,共6页Journal of Kunming Medical University

基　　金：国家自然科学基金资助项目(81820092)。

摘　　要：目的探讨microRNA-146a(miR-146a)与冠心病斑块稳定性的相关性。方法共收集胸痛患者324例。采用实时聚合酶链反应(Real-time PCR)检测miR-146a的表达。ELISA法检测hs-CRP、IL-6、IL-8水平。采用冠脉造影评价冠状动脉狭窄程度。应用血管内超声(IVUS)评价冠状动脉狭窄病变斑块稳定性。结果(1)UAP组和AMI组hs-CRP、IL-6、IL-8水平较CPS组明显升高(P<0.01);(2)冠心病患者外周血单个核细胞(PBMCs)和血浆miR-146a表达明显高于非冠心病组(P<0.05,P<0.001);(3)miR-146a在SAP组、UAP组和AMI组的表达高于CPS组(P<0.01,P<0.001,P<0.001),miR-146a在血浆中的表达模式与PBMCs一致;(4)易损斑块组外周血单个核细胞和血浆miR-146a的表达明显高于稳定斑块组(P<0.05);(5)软斑块组PBMCs和血浆miR-146a水平显著高于纤维斑块组和钙化斑块组(P<0.05)。结论miR-146a的表达与冠状动脉狭窄病变斑块稳定性密切相关。Objectives To investigate the association between microRNA-146a(miR-146a)and the plaque stability in patients with coronary heart disease.Methods Real-time PCR was used to measure miR-146a expression in 324 patients.ELISA was used to measure the level of hs-CRP,IL-6and IL-8.Coronary angiography was used to evaluate the degree of coronary artery stenosis.Intravascular ultrasound(IVUS)was used to evaluate the plaque stability of coronary stenotic lesions.Results(1)The levels of hs-CRP,IL-6 and IL-8 were significantly increased in the UAP and AMI groups compared with the CPS groups(P<0.01).(2)The miR-146a expression in peripheral blood mononuclear cells(PBMCs)and plasma was significantly higher in CAD patients than those in nonCAD group(P<0.05,P<0.001).(3)The miR-146a expression in PBMCs was higher in SAP,UAP and AMI group than CPS group(P<0.01,P<0.001,P<0.001).The expression patterns of miR-146a in plasma was consistent with that of PBMCs.(4)The expression of miR-146a in PBMCs and plasma were significantly higher in vulnerable plaque group than those in stable plaque group(P<0.05).(5)The level of miR-146a in PBMCs and plasma was significantly higher in soft plaque group than those in fibrous plaque group and calcified plaque group(P<0.05).Conclusions The expression of miR-146a is associated with the plaque stability of coronary stenotic lesions.

关 键 词：MIR-146A 冠心病 血管内超声 斑块稳定性 

分 类 号：R541.6[医药卫生—心血管疾病]