重庆市江津区小学生芽囊原虫感染情况及其危险因素  

Prevalence and risk factors of Blastocystis infections among primary school students in Jiangjin District,Chongqing City

作　　者：宁超群 康纪明 李伊婷 陈慧慧 储言红[1] 俞英昉[1] 吴秀萍[1] 艾琳[1] 陈家旭[1] 田利光[1] 廖启东 NING Chao-Qun;KANG Ji-Ming;LI Yi-Ting;CHEN Hui-Hui;CHU Yan-Hong;YU Ying-Fang;WU Xiu-Ping;AI Lin;CHEN Jia-Xu;TIAN Li-Guang;LIAO Qi-Dong(National Institute of Parasitic Disease,Chinese Center for Disease Control and Prevention,Chinese Center for Tropical Disease Research,WHO Collaborating Center for Tropical Diseases,National Center for International Research on Tropical Diseases,Ministry of Science and Technology,Key Laboratory of Parasites and Vector Biology,National Health Commission,Shanghai 200025,China;Jiangjin District Center for Disease Control and Prevention,Chongqing City,China)

机构地区：[1]中国疾病预防控制中心寄生虫病预防控制所、WHO热带病合作中心、科技部国家级热带病国际联合研究中心、国家卫生健康委员会寄生虫病原与媒介生物学重点实验室,上海200025 [2]重庆市江津区疾病预防控制中心

出　　处：《中国血吸虫病防治杂志》2020年第5期489-497,共9页Chinese Journal of Schistosomiasis Control

基　　金：国家自然科学基金(81473022)。

摘　　要：目的了解重庆市江津区小学生芽囊原虫感染率及其危险因素。方法2018年4月,采用横断面调查方法对重庆市江津区某小学在校学生进行问卷调查,并采集其粪便样本进行镜检、体外培养及PCR检测,以了解当地学生芽囊原虫感染情况及基因型分布。采用单因素分析和logistic回归分析对该校学生芽囊原虫感染危险因素进行分析。结果共调查466名小学生,平均年龄(9.81±1.66)岁,男性236例(50.64%)、女性230例(49.36%)。小学生芽囊原虫感染率为15.24%(71/466),其中男生感染率为16.52%(39/236)、女生为13.91%(32/230),差异无统计学意义(χ^2=0.616,P=0.433)。1~5年级小学生芽囊原虫感染率分别为6.35%(4/63)、5.17%(3/58)、21.74%(15/69)、25.30%(21/83)、10.19%(11/108)和20.00%(17/85),不同年级小学生芽囊原虫感染率差异有统计学意义(χ^2=15.410,P=0.009)。该校小学生感染的芽囊原虫基因型有4种(ST1、ST3、ST6和ST7);其中ST6型最多,占45.07%(32/71);其次是ST3型,占25.35%(18/71)。多因素logistic回归分析结果显示,少数民族[比值比(odds ratio,OR)=4.259,95%可信区间(confidential inter-val,CI):(1.161,15.621)]和母亲小学及以下受教育程度[OR=9.038,95%CI:(1.125,72.642)]是小学生芽囊原虫感染的危险因素。结论重庆市江津区小学生芽囊原虫感染率较高,感染的主要基因型为ST6和ST3,少数民族、母亲小学及以下受教育程度是该人群芽囊原虫感染的危险因素。Objective To investigate the prevalence and risk factors of Blastocystis infections among primary school students in Jiangjin District,Chongqing City.Methods A cross-sectional questionnaire survey was conducted among students sampled from a primary school in Jiangjin District,Chongqing City on April,2018,and their stool samples were collected for microscopic examinations,in vitro culture and PCR assays to analyze the prevalence of Blastocystis infections and subtype of the parasite.In addition,the risk factors of Blastocystis infections among primary school students were identified using univariate analysis and multivariate logistic regression analysis.Results A total of 466 primary students were surveyed,and the subjects had a mean age of(9.81±1.66)years and included 236 males(50.64%)and 230 females(49.36%).The prevalence of Blastocystis infections was 15.24%(71/466)among the study students,and there was no significance difference in the prevalence between male and female students(16.52%vs.13.91%;χ^2=0.616,P=0.433).In addition,there was a significant difference in the prevalence of Blastocystis infections among grade 1(6.35%,4/63),grade 2(5.17%,3/58),grade 3(21.74%,15/69),grade 4(25.30%,21/83),grade 5(10.19%,11/108)and grade 6 students(20.00%,17/85)(χ^2=15.410,P=0.009).There were four Blastocystis subtypes characterized(ST1,ST3,ST6 and ST7),in which ST6 was the most common subtype(45.07%,32/71),followed by ST3(25.35%,18/71).Multivariate logistic regression analysis revealed that minority ethnicity[odds ratio(OR)=4.259,95%confidential interval(CI):(1.161,15.621)]and low maternal education level(primary school and below)[OR=9.038,95%CI:(1.125,72.642)]were identified as risk factors of Blastocystis infection among primary school students in Jiangjin District,Chongqing City.Conclusions There is a high prevalence of Blastocystis infections detected among primary school students in Jiangjin District,Chongqing City,and ST6 and ST3 are predominant subtypes.Minority ethnicity and low maternal education level(primary s

关 键 词：芽囊原虫 基因型 危险因素 小学生 江津区 

分 类 号：R382.9[医药卫生—医学寄生虫学]