兔感染戊型肝炎病毒(HEV)对兔出血综合征(RHD)疫苗免疫效果的影响  

RHDV immunogenicity in rabbits infected with HEV

作　　者：金明兰[1,2,3] 王欢 郝心瑞[1] 李华南 杜志威[1] 徐莹莹 董莉莉[1] 薛洪海[1] 孙世梅[1] 李明[1] 王悦红[1] 金宁一 JIN Ming-lan;WANG Huan;HAO Xin-rui;LI Hua-nan;DU Zhi-wei;XU Ying-ying;DONG Li-li;XUE Hong-hai;SUN Shi-mei;LI Ming;WANG Yue-hong;JIN Ning-yi(Key Labora-tory of Songliao Aquatic Environment,Ministry of Education,Jilin Jianzhu University,Chang-chun 130118,China;School of Chemistry and Chemical Engineering,Shandong University,Jinan 250100,China;Institute of Military Veterinary Medicine,Academy of Military Medical Sci-ences,Changchun 130122,China)

机构地区：[1]吉林建筑大学松辽流域水环境教育部重点实验室,吉林长春130118 [2]山东大学化学与化工学院,山东济南250100 [3]军事医学科学院军事兽医研究所,吉林长春130122

出　　处：《中国兽医学报》2020年第10期1965-1969,共5页Chinese Journal of Veterinary Science

基　　金：长春市科技局资助项目(201801028JC);吉林省科技厅资助项目(20170101205JC,20180623026TC);安全生产监督部重大科研资助项目(jilin-0048-2018AQ);吉林省安全生产监督管理局重大科研资助项目(吉安监规划[2017]287);大学生创新创业资助项目(202010191070)。

摘　　要：为评估兔感染戊型肝炎病毒(HEV)对兔出血综合征(RHD)疫苗免疫效果的影响,将未知感染HEV的兔接种RHD疫苗,对其产生的RHD特异性抗体水平、丙氨酸转氨酶(ALT)、淋巴细胞增殖、IL-2、IFN-γ及HEV基因残留进行了检测。结果显示,兔产生兔出血综合征病毒(RHDV)和HEV的抗体水平较低;血清中ALT含量显著升高;淋巴细胞增殖、IL-2、IFN-γ的水平降低;接种疫苗后7,14 d检测到HEV基因,而21 d未检测到该病毒基因。结果表明,HEV在兔体内残留时间较长,对RHDV免疫水平具有一定的干扰作用。To evaluate the immunogenicity of RHDV in rabbits infected with HEV,rabbits were chosen to inoculate with RHDV alone.The RHDV-specific antibody,the proliferation of lymphocytes,contents of IL-2,IFN-γ and HEV gene residual were investigated respectively.During the experiment,the mental status,eating and drinking behaviors of the rabbits were un-normal.The experimental results showed that both specific humoral and cell-mediated immune responses could not be induced in animals immunized with RHDV.In particular,ALT showed the excellent increase,while the proliferation of lymphocytes,contents of IL-2 and IFN-γ could not increase significantly.In addition,viral nucleic acids were detected in liver at 7 and 14 day post inoculation(DPI).None viral nucleic acids could be detected from any organs at 21 DPI.It was evident that HEV were remained for a long time in rabbits,and interfered with the immune level of RHDV,thus provides valuable support for further development of RHDV genetic vaccines.

关 键 词：RHDV HEV ALT 免疫干扰 

分 类 号：S852.65[农业科学—基础兽医学] R535[农业科学—兽医学]