盐酸多西环素壳聚糖微球的制备与评价  

Preparation and evaluation of doxycycline chitosan microspheres

作　　者：王洪利 孙钰[2] 郝良玉 邵琳 王玥 易乐[2] 曲晗 王凯[2] 李乾学[2] 韩德明[1] WANG Hong-li;SUN Yu;HAO Liang-yu;SHAO Lin;WANG Yue;YI Le;QU Han;WANG Kai;LI Qian-xue;HAN De-ming(College of Life Science and Technology,Changchun University of Science and Technology,Changchun 130022,China;Institute of Mili-tary Veterinary Medicine,Academy of Military Medical Sciences,Changchun 130122,China;Jilin Agricultural University,Changchun 130000,China)

机构地区：[1]长春理工大学生命科学技术学院,吉林长春130022 [2]军事医学科学院军事兽医研究所,吉林长春130122 [3]吉林农业大学,吉林长春130000

出　　处：《中国兽医学报》2020年第10期2026-2031,共6页Chinese Journal of Veterinary Science

基　　金：国家重点研发计划资助项目(2016YFD0501000)。

摘　　要：制备盐酸多西环素(doxycycline hydrochloride,DH)缓释微球,并对其进行评价。通过Design-expert软件进行试验设计,以载药量和包封率为考察标准进行优化,采用乳化交联的方法制备壳聚糖包载DH缓释微球,运用紫外-可见分光光度计(UV-VIS Spectrophotometer)、扫描电子显微镜(SEM)、傅里叶变换红外光谱(FT-IR)、热重分析(TGA)、X射线衍射(XRD)及拉曼光谱(Raman spectra)对微球的结构、性能和形态进行评价。结果显示,在最佳制备条件下,即壳聚糖为20 g/L,体系内DH质量为0.3 g,转速为910 r/min,液体石蜡12 mL时,所制备载药微球的载药量为56.49%,包封率为61.41%。FT-IR表明壳聚糖包载DH主要以物理作用为主;热失重表明微球物理包合后热稳定性较差;XRD结果表明DH被包载后晶体结构未发生变化。结果表明,本试验成功制备表面光滑、粒径整齐、载药量和包封率较高的DH壳聚糖微球。Preparation and evaluation of doxycycline hydrochloride(DH) sustained-release microspheres.The content and entrapment efficiency were used as the main index of examimation,and orthogonal design was used to optimize the doxycycline hydrochloride-chitosan microcapheres on the base of Design-expert software.The emulsion cross-linking method was used to prepare chitosan-loaded doxycycline sustained-release microcapheres.The doxycycline hydrochloride-chitosan microcapheres were evaluation by visible spectrophotometer(UV-VIS spectrophotometer),scanning electron microscope(SEM),Fourier transform infrared spectroscopy(FT-IR),thermogravimetric analysis(TGA),X-ray diffraction(XRD) and Raman spectroscopy(Raman Spectra).Based on optimal preparation conditions,the concentration of chitosan was 20 g/L,the mass of doxycycline hydrochloride was 0.3 g,the rotation speed was 910 r/min,and the volume of liquid paraffin was 12 mL.56.49% and 61.41% were got as the mean drug loading and entrapment efficiency.Infrared spectroscopy indicated that chitosan-loaded doxycycline hydrochloride was mainly composed of physical action;thermal weight loss indicated that the thermal stability of microspheres after physical inclusion was poor;X-ray diffraction results showed that after doxycycline hydrochloride was encapsulated,the crystal structure has not changed.The doxycycline chitosan microspheres with smooth surface,uniform particle size,high drug loading and encapsulation efficiency were successfully prepared.

关 键 词：盐酸多西环素 壳聚糖微球 包封率 载药量 

分 类 号：S859.79[农业科学—临床兽医学]