艾灸对类风湿性关节炎模型大鼠足跖滑膜组织PI3K/Akt/mTOR信号通路的影响  

Effect of moxibustion on PI3K/Akt/mTOR signaling pathway in foot-pad synovium in rats with rheumatoid arthritis

作　　者：郝锋 吴立斌 胡骏 刘磊 吴子建 蔡荣林 胡玲 杨小存 王洁 余情 何璐 HAO Feng;WU Li-bin;HU Jun;LIU lei;WU Zi-jian;CAI Rong-lin;HU Ling;YANG Xiao-cun;WANG Jie;YU Qing;HE Lu(College of Acupuncture-Moxibustion and Tuina,Nanjing University of Chinese Medicine,Nanjing 210023,Jiangsu Province,China;College of Acupuncture-Moxibustion and Tuina,Anhui University of Chinese Medicine;First Clinical Medical College of Nanjing University of Chinese Medicine;Institute of Acupuncture-Moxibustion and Meridian,Anhui Academy of TCM,Hefei 230038)

机构地区：[1]南京中医药大学针灸推拿学院,江苏南京210023 [2]安徽中医药大学针灸推拿学院 [3]南京中医药大学第一临床医学院 [4]安徽省中医药科学院针灸经络研究所,合肥230038

出　　处：《中国针灸》2020年第11期1211-1216,共6页Chinese Acupuncture & Moxibustion

基　　金：江苏省高等学校自然科学研究面上项目:18KJB360005;国家重点基础研究发展计划项目:2015CB554504;安徽中医药大学探索科研项目:2016ts024。

摘　　要：目的:观察艾灸对类风湿性关节炎(RA)模型大鼠足跖滑膜组织磷脂酰肌醇3-激酶/蛋白激酶B/哺乳动物雷帕霉素靶蛋白(PI3K/Akt/mTOR)信号通路的影响,探索艾灸治疗RA的作用机制。方法:将40只健康SD大鼠随机分为对照组、模型组、艾灸组、香烟灸组和药物组,每组8只。除对照组外,其余4组采用风、寒、湿环境因素结合左后足跖皮下注射完全弗氏佐剂(FCA)建立RA模型。艾灸组予艾灸左侧"足三里"20 min;香烟灸组以普通卷烟灸左侧"足三里"20 min;药物组予雷公藤多苷片混悬液(0.8 mg/100 g)灌胃。均每日1次,共干预15 d。造模前后及干预15 d后测量左后足跖容积;干预15 d后采用ELISA法检测血清白介素(IL)-17、IL-23含量,Westernblot法检测左后足跖滑膜组织PI3K、Akt、mTOR蛋白表达水平。结果:干预后模型组大鼠左后足跖容积,血清IL-23、IL-17含量及左后足跖滑膜组织PI3K、Akt、mTOR蛋白表达水平均高于对照组(P<0.01);艾灸组和药物组大鼠干预后左后足跖容积和滑膜组织PI3K、Akt、mTOR蛋白表达水平均低于模型组(P<0.01,P<0.05),艾灸组血清IL-17、IL-23含量低于模型组(P<0.01);艾灸组大鼠左后足跖容积、血清IL-23含量与左后足跖滑膜组织mTOR蛋白表达水平低于药物组(P<0.01,P<0.05),艾灸组左后足跖容积、血清IL-23含量与左后足跖滑膜组织PI3K、Akt、m TOR蛋白表达水平低于香烟灸组(P<0.01)。结论:艾灸可能通过抑制IL-23、IL-17水平与PI3K/Akt/mTOR信号通路活性,调节炎性反应与自噬活动,发挥对RA的治疗效应。Objective To observe the effect of moxibustion on phosphatidylinositol 3-kinase/protein kinase B/mammalian target of rapamycin protein(PI3K/Akt/mTOR) signaling pathway in the foot-pad synovial tissue in rats with rheumatoid arthritis(RA), and to explore the mechanism of moxibustion for treating RA. Methods Forty healthy SD rats were randomly divided into a control group, a model group, a moxibustion group, a cigarette-moxibustion group and a medication group, 8 rats in each group. The RA model was established with subcutaneous injection of complete Freund’s adjuvant(CFA) in the left hind foot-pad under wind, cold and wet environment in the model group, the moxibustion group, the cigarette-moxibustion group and the medication group. The rats in the moxibustion group were treated with moxibustion at "Zusanli"(ST 36) for20 min;the rats in the cigarette-moxibustion group were treated with moxibustion of ordinary cigarette at "Zusanli"(ST 36) for20 min;the rats in the medication group were treated with tripterygium glycosides suspension(0.8 mg/100 g) by gavage. All the intervention was given once a day for 15 days. The left hind foot-pad volume was measured before and after modeling and after 15-day intervention. After 15-day intervention, the serum levels of IL-17 and IL-23 were detected by ELISA method, and the expression levels of PI3K, Akt and mTOR in synovial tissue of left hind foot-pad were detected by Western blot method.Results The volume of left hind foot-pad, the serum levels of IL-23 and IL-17 and the expression of PI3K, Akt and mTOR in synovial tissue of left hind foot-pad in the model group were higher than those in the control group(P<0.01). After intervention, the volume of left hind foot-pad and the expression of PI3K, Akt and mTOR protein in synovium tissue in the moxibustion group and medication group were lower than those in the model group(P<0.01, P<0.05), while the serum levels of IL-17 and IL-23 in the moxibustion group were lower than those in the model group(P<0.01). The volume of left hind

关 键 词：类风湿性关节炎 艾灸 炎性反应 细胞自噬 PI3K/Akt/mTOR通路 

分 类 号：R245.81[医药卫生—针灸推拿学]