毛细管电泳在中药糖类成分分析中的研究进展  

Research advance of capillary electrophoresis on the analysis of saccharides from traditional Chinese medicine

作　　者：刘美廷 赵静[1] 李绍平[1] LIU Mei-ting;ZHAO Jing;LI Shao-ping(State Key Laboratory of Quality Research in Chinese Medicine,University of Macao,Macao 999078,China)

机构地区：[1]澳门大学中药质量研究国家重点实验室,中国澳门999078

出　　处：《药物分析杂志》2020年第10期1727-1735,共9页Chinese Journal of Pharmaceutical Analysis

基　　金：国家自然科学基金(No.81673389);国家重点研发计划(2019YFC1711300);广东省重点领域研发计划(2020B1111110006);澳门科学技术发展基金项目(0075/2018/A2,034/2017/A1和0017/2019/AKP);澳门大学科学研究基金项目(MYRG2018-00083-ICMS,MYRG2019-00128-ICMS和CPG2020-00021-ICMS)。

摘　　要：糖类成分在中药广泛分布,具有多种药理活性。毛细管电泳法(capillary electrophoresis,CE)具备分离效率高、样品消耗量少等优势,在糖类分析中得到较好的应用。本文对CE在中药糖类成分分析中的应用进行了总结。同时介绍了CE针对糖类分析的分离策略和检测方法的研究进展,分离策略包括糖类与硼酸根络合或在强碱条件下电离等,检测方法包括紫外检测、荧光检测、电化学检测和质谱检测。以期为CE在中药糖类成分分析中的研究提供参考。Saccharides are widely distributed in traditional Chinese medicine and have lots of pharmacological activities. Capillary electrophoresis(CE) has the advantages of high resolution and less sample consumption. This review summarized the applications of CE on analysis of saccharides from traditional Chinese medicine. Meanwhile the research advance of CE isolation strategies and detection methods were introduced. The isolation strategies included saccharides complexing with borate or isolating in highly alkaline buffer and so on. And the detection methods were based on ultraviolet,laser induced fluorescence,electrochemistry and mass spectrometry. It was expected that this review could provide references for the research on CE analysis of saccharides from traditional Chinese medicine.

关 键 词：毛细管电泳 中药 单糖 寡糖 多糖 成分分析 

分 类 号：R917[医药卫生—药物分析学]