滋阴活血利水合剂对大鼠脑缺血再灌注损伤的保护及增强脑组织抗氧化能力的机制研究  

Protective Effect of Ziyin Huoxue Lishui Mixture on Cerebral Ischemia-reperfusion Injury in Rats and Its Mechanism of Enhancing Antioxidant Capacity of Brain Tissue

作　　者：陈照宇 庄丽 韩光明[1] 郑昆[1] 刘安信 Chen Zhaoyu;Zhuang Li;Han Guangming;Zheng Kun;Liu Anxin(Rizhao Hospital of Traditional Chinese Medicine,Shandong Rizhao 276000,China)

机构地区：[1]日照市中医医院,山东日照276000

出　　处：《中国药师》2020年第11期2099-2102,2108,共5页China Pharmacist

摘　　要：目的:观察滋阴活血利水合剂对大鼠脑缺血再灌注损伤的保护作用,并探讨其可能的作用机制。方法:90只大鼠采用体质量区组化随机分为6组:假手术组、模型组、血塞通组(阳性对照,54 mg·kg^-1)、滋阴活血利水合剂高、中、低剂量组(45,22.5,11.25 ml·kg^-1),每组15只。采用线栓法制作大鼠大脑中动脉缺血后再灌注损伤模型,各组均灌胃给药,3次/d,连续15 d,假手术组和模型组给予等体积生理盐水。观察治疗后大鼠的脑梗死体积变化、脑组织病理形态及脑组织中超氧化物歧化酶(SOD)、丙二醛(MDA)、羟自由基(·OH)、谷胱甘肽过氧化物酶(GSH-Px)、总抗氧化能力(T-AOC)等的含量变化。结果:与模型组比较,滋阴活血利水合剂各剂量组和血塞通组大鼠的脑梗死体积比、MDA和·OH含量均显著降低(P<0.05),SOD、GSH-Px及T-AOC含量均显著升高(P<0.05),且滋阴活血利水合剂呈现剂量依赖性;与血塞通组比较,滋阴活血利水合剂高剂量组大鼠的脑组织梗死体积比、MDA和·OH含量均显著降低(P<0.05),SOD、GSH-Px及T-AOC含量均显著升高(P<0.05),而中、低剂量组差异无统计学意义(P>0.05)。结论:滋阴活血利水合剂对脑缺血再灌注损伤模型大鼠脑组织具有明显的保护作用,能够显著缩小脑梗死体积,改善脑水肿,其作用机制可能与增强脑组织内抗氧化能力相关。Objective:To observe the protective effects of Ziyin Huoxue Lishui mixture on cerebral ischemia reperfusion injury in rats and explore its possible mechanism.Methods:Totally 90 rats were randomly divided into 6 groups:sham operation group,model group,Xuesaitong positive control group(54 mg·kg^-1),Ziyin Huoxue Lishui mixture high,medium and low dose groups(45,22.5,11.25 ml·kg^-1)with 15 rats in each group.Ischemia reperfusion injury model was duplicated by thread embolism method.The rats in each treated group were given corresponding drug by gavage for 15 days,3 times a day,and the sham operation group and the model group were given equal volume of normal saline.The changes of cerebral infarction volume,pathological morphology of brain tissue and levels of SOD,MDA,·OH,GSH-Px and T-AOC in brain tissue after treatment were evaluated.Results:Compared with those in the model group,the volume of cerebral infarction and the levels of MDA and·OH in the positive control group,Ziyin Huoxue Lishui mixture high,medium and low dose groups significantly decreased(P<0.05),while the levels of SOD,GSH-Px and T-AOC increased obviously(P<0.05).Moreover,there was a dose-dependent manner in Ziyin Huoxue Lishui mixture groups.Compared with those in the positive control group,the volume of cerebral infarction and the levels of MDA and·OH in Ziyin Huoxue Lishui mixture high dose group decreased markedly(P<0.05),while the levels of SOD,GSH-Px and T-AOC increased dramatically(P<0.05).However,no significant difference was found in the medium or low dose group(P>0.05).Conclusion:Ziyin Huoxue Lishui mixture shows obvious protective effects on brain tissue of cerebral ischemia reperfusion injury,which can significantly reduce the volume of cerebral infarction and relieve brain edema.Its mechanism may be related to the enhancement of antioxidant capacity in brain tissue.

关 键 词：滋阴活血利水合剂 脑缺血再灌注损伤模型 抗氧化 

分 类 号：R285.5[医药卫生—中药学]