UPLC法同时测定小建中颗粒中8种有效成分  

Simultaneous Determination of Eight Components in Xiaojianzhong Granules by UPLC

作　　者：黄亚南 曾诚[1] 胡梦楚 黄湘杰 高玉松 Huang Ya'nan;Zeng Cheng;Hu Mengchu;Huang Xiangjie;Gao Yusong(Gynecology Department,the First Affiliated Hospital of Guangzhou Traditional Chinese Medicine University,Guangzhou 510405,China;Pharmacy Department of Xiangxiang People's Hospital;Pharmacy Center,Beijing Rehabilitation Hospital of Capital Medical University Rehabilitation)

机构地区：[1]广州中医药大学第一附属医院妇科,广州510405 [2]湖南省湘乡市人民医院药剂科 [3]首都医科大学附属北京康复医院药学中心

出　　处：《中国药师》2020年第11期2287-2290,共4页China Pharmacist

摘　　要：目的:建立同时测定小建中颗粒中芍药苷、甘草苷、香豆素、肉桂酸、甘草酸铵、桂皮醛、6-姜辣素和甘草次酸含量的方法。方法:以L9(34)正交试验优化提取方式,采用ACQUITY UPLC HSS C18色谱柱(100 mm×2.1 mm,1.8μm),以乙腈-0.1%磷酸溶液为流动相,流速为0.6 ml·min^-1,梯度洗脱,柱温为25℃,检测波长为254 nm。结果:小建中颗粒的最佳提取方式为60%乙醇25 ml超声提取30 min。芍药苷、甘草苷、香豆素、肉桂酸、甘草酸铵、桂皮醛、6-姜辣素、甘草次酸的线性范围分别为7.10~709.60μg·ml^-1(r=0.9997)、2.78~278.20μg·ml^-1(r=0.9994)、0.19~18.80μg·ml^-1(r=0.9982)、0.40~39.80μg·ml^-1(r=0.9989)、1.59~159.20μg·ml^-1(r=0.9992)、2.44~244.20μg·ml^-1(r=0.9994)、2.86~285.60μg·ml^-1(r=0.9996)、0.32~32.40μg·ml^-1(r=0.9986);加样回收率的范围为98.1%~102.2%;精密度、稳定性、重复性试验RSD均小于2.0%。结论:本方法稳定可靠,重复性好,可为小建中颗粒的质量控制提供参考。Objective:To establish a method for the simultaneous determination of paeoniflorin,liquiritin,coumarin,cinnamic acid,ammonium glycyrrhizinate,cinnamaldehyde,6-gingerol and glycyrrhetinic acid in Xiaojianzhong granules.Methods:The L9(34)orthogonal design was carried out in order to optimize the extraction method.The analysis was performed on an ACQUITY UPLC HSS C18 chromatographic column(100 mm×2.1 mm,1.8μm)with acetonitrile and 0.1%phosphoric acid as the mobile phase with gradient elution.The flow rate was 0.6 ml·min^-1,the column temperature was 25℃,and the wavelength was 254 nm.Results:The best extraction method of Xiaojianzhong granules was 60%ethanol 25 ml ultrasonic extraction for 30 mins.The linear range of paeoniflorin,liquiritin,coumarin,cinnamic acid,ammonium glycyrrhizinate,cinnamaldehyde,6-gingerol and glycyrrhetinic acid was 7.10-709.60μg·ml^-1(r=0.9997),2.78-278.20μg·ml^-1(r=0.9994),0.19^-18.80μg·ml^-1(r=0.9982),0.40-39.80μg·ml^-1(r=0.9989),1.59^-159.20μg·ml^-1(r=0.9992),2.44-244.20μg·ml^-1(r=0.9994),2.86-285.60μg·ml^-1(r=0.9996)and 0.32-32.40μg·ml^-1(r=0.9986),respectively.The range of recoveries was between 98.1%and 102.2%.The RSDs of precision,stability and repeatability were all less than 2.0%.Conclusion:This method is stable and repeatable,and can be used for the quality control of Xiaojianzhong granules.

关 键 词：小建中颗粒 超高效液相色谱法 芍药苷 甘草苷 香豆素 肉桂酸 甘草酸铵 桂皮醛 6-姜辣素 甘草次酸 含量测定 

分 类 号：R927.2[医药卫生—药学]