日本血吸虫天然抗病分子疫苗的结构与功能分析II.SIEA 28kDa分子的高效液相色谱分离及薄层等电聚焦分析  

Isolating and Analyzing of the SIEA 28 kDa Component of Schistosoma Japonicum by High Performance Liquid Chromatography and Thin-layer PAGIEF

作　　者：汪世平[1] 谭耀武[1] 刘立鹏[1] 徐绍锐[1] 

机构地区：[1]中南大学基础医学院,长沙410078

出　　处：《科学技术与工程》2002年第5期26-28,共3页Science Technology and Engineering

基　　金：国家自然科学基金(39870652)资助;卫生部"全国地方病防治跨世纪科技优秀人才"及"湖南省重点学科建设项目资助"费资助。

摘　　要：采用高效液相离子交换法对电泳纯SIEA 28 kDa抗原组分进一步分离纯化。对获得的色谱纯SIEA 28 kDa抗原分子进行薄层等电聚焦分析,测定其等电点。结果证明电泳纯SIEA 28 kDa抗原分子经高效液相离子交换法纯化后,280nm、220nm波长色谱图均为一个两侧对称、光滑的高峰曲线,色谱洗脱液对其免疫活性光密度图也为一个两侧对称、光滑的高峰曲线。色谱纯化证明SIEA 28 kDa抗原组分可能为单一分子,其等电点范围在p15-p17之间。The SIEA 28 kDa component of Schistosoma japonicum purified by gel electrophoresis was isolated and purified further by ion exchange high performance liquid chromatography (HPLC) and then its pI was measured by thin-layer PAG-IEF. After purified by HPLC, its component took the appearance of a symmetric smooth chromatography curve with a single peak both in 220 nm and 280 nm wavelength. The curve of chromatography elusion verse its activity was also symmetric and single-peaked. The result shows that SIEA 28 kDa component is probably a single molecule and its pI is ranged from 5 to 7.

关 键 词：天然抗病分子疫苗 功能分析 薄层等电聚焦分析 日本血吸虫 SIEA28kDa抗原 高效液相色谱法 结构分析 

分 类 号：R383.24[医药卫生—医学寄生虫学] R392[医药卫生—基础医学]