平贝母鳞茎腐烂病病原菌鉴定和药剂筛选  

Identification of the pathogen of the bulb rot of Fritillaria ussuriensis and screening of the fungicides

作　　者：王爽[1] 李新民[1] 刘春来[1] 杨帆[1] 刘亮 刘博 WANG Shuang;LI Xinmin;LIU Chunlai;YANG Fan;LIU Liang;LIU Bo(Plant Protection Institute,Heilongjiang Academy of Agricultural Sciences,Harbin 150086,China;College of Plant Protection,China Agricultural University,Beijing 100193,China)

机构地区：[1]黑龙江省农业科学院植物保护研究所,哈尔滨150086 [2]中国农业大学植物保护学院,北京100193

出　　处：《植物保护》2020年第6期65-70,共6页Plant Protection

基　　金：国家重点研发计划(2017YFD0201108-04);黑龙江省农业科学院“农业科技创新跨越工程”经济作物科技创新专项(HNK2019CX06-01-03)。

摘　　要：为明确平贝母Fritillaria ussuriensis鳞茎腐烂病的病原菌及其防治,对病原菌进行分离、致病性测定,通过形态学特征、ITS rDNA、β-tubulin及EF-1α基因序列分析确定其病原为尖孢镰刀菌Fusarium oxysporum和芳香镰刀菌F.redolens。通过菌丝生长速率法,对病原菌进行了室内药剂筛选,表明8种药剂中25%多菌灵可湿性粉剂和43%戊唑醇悬浮剂对病原菌有更好的抑制作用,处理第6天,EC 50均不超过0.7 mg/L。对两种病原菌防效最好的药剂是戊唑醇,EC 50分别为0.107、0.169 mg/L。这两种药剂可以选择应用于平贝母生产来防治茎腐病。To make clear the fungal pathogen species of the bulb rot of Fritillaria ussuriensis and the control,pathogens were isolated and their pathogenicities were tested.Based on morphological characteristics,ITS rDNA,β-tubulin and EF-1αgene sequences analysis,pathogens were identified were Fusarium oxysporum and F.redolens.Indoor inhibitory activity analysis of eight kinds of fungicides demonstrated that two kinds of fungicides including carbendazim 25%WP and tebuconazole 43%SC had better inhibitory effect on two pathogens.With EC 50 values no more than 0.7 mg/L.Results showed that tebuconazole was the best agent to inhibit the mycelia of the two pathogens,with the EC 50 values were 0.107 mg/L and 0.169 mg/L,respectively.Thus,these two kinds of fungicides can be recommended as alternative agents for field testing.

关 键 词：平贝母 茎腐病 尖孢镰刀菌 芳香镰刀菌 药剂筛选 

分 类 号：S435.67[农业科学—农业昆虫与害虫防治]