澧县‘红地球’葡萄表面黑色组曲霉种群结构及产毒情况研究  

Population structure and toxigenicity profiling of Aspergillus section Nigri on the surface of grape‘Red Earth’in Lixian county

作　　者：页非 黄晓庆[2] 王忠跃[2] 孔繁芳[2] 陈湘云[1] 杨国顺[1] 张昊[2] YE Fei;HUANG Xiaoqing;WANG Zhongyue;KONG Fanfang;CHEN Xiangyun;YANG Guoshun;ZHANG Hao(College of Horticulture and Landscape,Hunan Agricultural University,Changsha 410128,China;State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193,China)

机构地区：[1]湖南农业大学园艺园林学院,长沙410128 [2]中国农业科学院植物保护研究所,植物病虫害生物学国家重点实验室,北京100193

出　　处：《植物保护》2020年第6期124-130,135,共8页Plant Protection

基　　金：国家重点研发计划(2018YFD0201300);国家现代农业产业技术体系建设专项(CARS-29)。

摘　　要：葡萄和葡萄制品面临着真菌毒素的威胁,这些真菌毒素主要来自于黑色组曲霉Aspergillus section Nigri。本研究对采自湖南省澧县‘红地球’葡萄上的黑色组曲霉进行了分离鉴定及种群结构分析,并采用超高效液相色谱串联质谱技术(ultra performance liquid chromatography-tandem mass spectrometry,UPLC-MS/MS)对12株代表菌株产赭曲霉毒素(ochratoxin A,OTA)和伏马毒素B 2(fumonisin B 2,FB 2)的能力进行了测定。结果表明,澧县地区‘红地球’葡萄带菌率为92%。基于钙调素序列,分离得到的131株黑色组曲霉分属于5个种,其中A.aculeatinus为优势种。12株代表菌株均不产生OTA;A.welwitschiae(4/5)及A.niger(2/2)可产生FB 2,平均产毒量分别为3.36 ng/g及741.37 ng/g。上述结果为评估国内葡萄表面黑色组曲霉及其毒素的污染风险提供参考。Grapes and grape products are threatened by mycotoxins,caused by some species of Aspergillus section Nigri.In this study,based on calmodulin gene sequencing,using ultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS),the Aspergillus section Nigri population and its toxigenicity in grapes in Lixian county of Hunan province were studied to confirm the food safety of table grapes.A total of 131 Aspergillus section Nigri strains were isolated from grape samples with the isolation frequency was 92%,and five species of Aspergillus section Nigri were identified,with A.aculeatinus as the dominant species.The mycotoxin tests of 12 isolated strains showed that none of them produced ochratoxin A.A.welwitschiae(4/5)and A.niger(2/2)could produce fumonisin B 2,with an average toxin yield of 3.36 ng/g and 741.37 ng/g,respectively.This study provided a reference for evaluating the occurrence of Aspergillus section Nigri and its toxigenicity on the surface of grapes in China.

关 键 词：葡萄 黑色组曲霉 赭曲霉毒素(OTA) 伏马毒素B 2(FB 2) 

分 类 号：S436.631.1[农业科学—农业昆虫与害虫防治]