根结线虫生防真菌交枝顶孢原生质体的制备与再生体系构建  

Preparation and regeneration of protoplasts from the biocontrol fungus Acremonium implicatum

作　　者：姚玉荣 霍建飞[1] 郝永娟[1] 王万立[1] YAO Yurong;HUO Jianfei;HAO Yongjuan;WANG Wanli(Tianjin Institute of Plant Protection,Tianjin 300381,China;Key Laboratory of Postharvest Physiology and Storage of Agricultural Products,Ministry of Agriculture and Rural Affairs,Tianjin 300384,China)

机构地区：[1]天津市植物保护研究所,天津300381 [2]农业农村部农产品贮藏保鲜重点实验室,天津300384

出　　处：《植物保护》2020年第6期149-154,共6页Plant Protection

基　　金：国家重点研发计划(2018YFD0201207);天津市青年基金(18JCQNJC14900);天津市农业科学院院长基金(17002)。

摘　　要：本研究以根结线虫生防真菌交枝顶孢Acremonium implicatum为供试菌株,探究了菌龄、降解酶种类、酶解温度和时间、不同渗透压稳定剂、不同pH等因素对其原生质体制备的影响,并建立了交枝顶孢原生质体制备体系;制备的原生质体在SR培养基上再生率达到17.78%。此外,GFP遗传转化菌株的获得充分表明制备的原生质体可作为后续遗传转化材料,为进一步研究交枝顶孢对根结线虫的生防机理奠定基础。In order to establish protoplast-mediated genetic transformation of the biocontrol fungus Acremonium implicatum,the conditions for the isolation and regeneration of A.implicatum protoplasts were optimized,including culture time,enzyme system,enzymatic digestion time and temperature,type of osmotic stabilizers,pH value and so on.For the preparation of protoplasts,solid regeneration medium was better with a regeneration rate of protoplasts of 17.78%.The transformant strain with GFP was obtained.The system may build a foundation for further study on the mechanism of biocontrol against the root-knot nematode.

关 键 词：交枝顶孢 原生质体制备 再生 绿色荧光蛋白 

分 类 号：S435.45[农业科学—农业昆虫与害虫防治] S476[农业科学—植物保护]