七氟菊酯和溴氰菊酯对棉铃虫肠道菌群的影响  

Effects of tefluthrin and deltamethrin on gut microbiota in Helicoverpa armigera (Lepidoptera: Noctuidae)

作　　者：姜笑维 关丹阳 李清亚 刘晓 Hongmei LI-BYARLAY 贺秉军[1] JIANG Xiao-Wei;GUAN Dan-Yang;LI Qing-Ya;LIU Xiao;Hongmei LI-BYARLAY;HE Bing-Jun(Key Laboratory of Bioactive Materials,Ministry of Education,College of Life Sciences,Nankai University,Tianjin 300071,China;Central State University,Wilberforce,OH 45384,USA)

机构地区：[1]南开大学生命科学学院,生物活性材料教育部重点实验室,天津300071 [2]Central State University,Wilberforce,OH 45384,USA

出　　处：《昆虫学报》2020年第10期1183-1193,共11页Acta Entomologica Sinica

基　　金：国家自然科学基金项目(31871992,31371974)。

摘　　要：【目的】本研究旨在探究拟除虫菊酯类杀虫剂对棉铃虫Helicoverpa armigera幼虫肠道菌群结构及代谢的影响,丰富对杀虫剂作用机理的认识。【方法】分别对棉铃虫2和3龄幼虫饲喂普通人工饲料(对照组,SS)、含2%七氟菊酯(Ⅰ型拟除虫菊酯)粉剂饲料(七氟菊酯处理组,Te)和含2.5%溴氰菊酯(Ⅱ型拟除虫菊酯)乳油饲料(溴氰菊酯处理组,DM),然后提取3龄幼虫肠道菌群基因组DNA;利用Illumina MiSeq二代高通量测序技术对肠道细菌的16S rDNA的V3-V4变异区进行测序,分析其肠道细菌的多样性和丰富度;利用qPCR验证16S rDNA测序分析结果。取2和3龄幼虫肠道,匀浆后进行Biolog-Eco实验,分析肠道细菌对Eco板上31种碳源的代谢情况。【结果】16S rDNA测序结果表明,棉铃虫3龄幼虫肠道细菌主要是厚壁菌门(Firmicutes)、变形菌门(Proteobacteria)、拟杆菌门(Bacteroidetes)和蓝藻菌门(Cyanobacteria)。与对照组相比,溴氰菊酯处理组和七氟菊酯处理组的棉铃虫幼虫肠道细菌的α多样性指数没有显著性改变,但是菌群结构发生了变化:在门水平,拟杆菌门的相对丰度减少,厚壁菌门和蓝藻菌门的相对丰度增加,qPCR验证结果亦支持16S rDNA测序分析的这个结果;在属水平,拟杆菌属Bacteroides、普氏菌属Prevotella和假单胞菌属Pseudomonas等的相对丰度降低,狭义梭菌属Clostridium sensu stricto 1、埃希菌属-志贺氏菌属Escherichia-Shigella和盐单胞菌属Halomonas等的相对丰度增加,其中盐单胞菌属Halomonas的相对丰度显著增加。Biolog-Eco结果表明,与对照组相比,溴氰菊酯处理组中2龄幼虫对羧酸类碳源的代谢能力下降;溴氰菊酯处理组和七氟菊酯处理组中3龄幼虫对DL-α-磷酸甘油、肝糖和L-苯丙氨酸等碳源的利用能力下降。【结论】结果显示,拟除虫菊酯类杀虫剂对棉铃虫肠道菌群的结构和代谢能力有明显影响,拟除虫菊酯类杀虫剂使棉铃虫肠道有益菌【Aim】This study aims to address the effects of pyrethroid insecticides on the structure and metabolism of larval gut microbiota of Helicoverpa armigera and to enrich the knowledge about the action mechanisms of pesticides.【Methods】The 2nd and 3rd instar larvae of H.armigera were fed with the normal artificial diet(control group,SS)and diets containing 2%tefluthrin(type I pyrethroid)powder(tefluthrin treatment group,Te)and 2.5%deltamethrin(type II pyrethroid)emulsifiable concentrate(deltamethrin treatment group,DM),respectively,and then the genomic DNA of gut bacteria of the 3rd instar larvae was extracted.Then the V3-V4 region of 16S rDNA of gut bacteria was sequenced using Illumina MiSeq of the 2nd generation high-throughput sequencing technology to analyze the diversity and abundance of gut bacteria,and qPCR was performed to verify the 16S rDNA sequencing and analysis results.In addition,the gut samples of the 2nd and 3rd instar larvae of H.armigera were milled for Biolog-Eco experiment to analyze the metabolism of 31 carbon sources by gut bacteria on the Eco plate.【Results】Sequencing results of 16S rDNA showed that the gut bacteria of the 3rd instar larvae of H.armigera were mainly composed of Firmicutes,Proteobacteria,Bacteroidetes and Cyanobacteria.Compared with the control group,theα-diversity index of the larval gut bacteria in the deltamethrin and tefluthrin treatment groups did not change significantly,but the community structure of bacteria changed.At the phylum level,the relative abundance of Bacteroides in the deltamethrin and tefluthrin treatment groups decreased as compared with that in the control group,while that of Firmicutes and Cyanobacteria increased.qPCR verification results supported the above 16S rDNA sequencing and analysis results.At the genus level,the relative abundance of Bacteroides,Prevotella,and Pseudomonas was reduced,and that of Clostridium sensu stricto 1,Escherichia-Shigella,and Halomonas increased,especially Halomonas with significant increase.Biolog-Eco results sh

关 键 词：棉铃虫 肠道细菌 菌群结构 16S rDNA Biolog-Eco 拟除虫菊酯 碳源 

分 类 号：Q965.8[生物学—昆虫学]