转cry1Ab/cry1Ac基因玉米Cry1Ab/Cry1Ac融合蛋白表达及对亚洲玉米螟的室内杀虫效果  

Expression of Cry1Ab/Cry1Ac fusion protein in the transgenic cry1Ab/cry1Ac maize and its control efficacy against the Asian corn borer,Ostrinia furnacalis(Lepidoptera:Crambidae),in the laboratory

作　　者：刘晓贝 白树雄[2] 王振营[2] 王月琴[2] 王勤英[1] 何康来[2] LIU Xiao-Bei;BAI Shu-Xiong;WANG Zhen-Ying;WANG Yue-Qin;WANG Qin-Ying;HE Kang-Lai(College of Plant Protection,Hebei Agricultural University,Baoding,Hebei 071000,China;State Key Laboratory for Biology of Plant Diseases and Insect Pests,Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193,China)

机构地区：[1]河北农业大学植物保护学院,河北保定071000 [2]中国农业科学院植物保护研究所,植物病虫害生物学国家重点实验室,北京100193

出　　处：《昆虫学报》2020年第10期1201-1206,共6页Acta Entomologica Sinica

基　　金：国家转基因生物新品种培育重大专项(2016ZX08003-001)。

摘　　要：【目的】室内抗螟性评价是转Bt基因抗虫玉米研发和安全性评价的重要环节。【方法】采用酶联免疫吸附测定法(ELISA)测定了转cry1Ab/cry1Ac基因玉米ZZM030心叶中Cry1Ab/Cry1Ac融合杀虫蛋白的表达量;采用室内生测法测定了分别取食转基因玉米ZZM030和非转基因玉米X249心叶后亚洲玉米螟Ostrinia furnacalis敏感品系ACB-BtS、Cry1Ab抗性品系ACB-AbR和Cry1Ac抗性品系ACB-AcR初孵幼虫的存活率。【结果】转基因抗虫玉米ZZM0304叶期和8叶期心叶中Cry1Ab/Cry1Ac融合杀虫蛋白的表达量分别是10.62和2.94μg/g FW。敏感品系亚洲玉米螟初孵幼虫取食转基因玉米ZZM030心叶2 d的存活率仅为23.6%,4 d后存活率为0,而取食非转基因对照玉米X249心叶4 d的存活率高达93.1%。Cry1Ab抗性品系和Cry1Ac抗性品系初孵幼虫取食转基因玉米ZZM030心叶6 d后的存活率分别为11.1%和12.5%,而取食非转基因玉米X249心叶6 d后的存活率分别为81.9%和77.8%。【结论】转cry1Ab/cry1Ac基因玉米ZZM030心叶中高表达的Cry1Ab/Cry1Ac融合蛋白对亚洲玉米螟初孵幼虫具有极高的杀虫效果。【Aim】Evaluating the efficacy of transgenic Bt maize for resistance to target insect in the laboratory is a key part of the research and development and the environmental safety assessment of insect resistant transgenic maize.【Methods】The expression levels of Cry1Ab/Cry1Ac fusion protein in fresh whorl leaves of transgenic cry1Ab/cry1Ac maize ZZM030 were quantified by using enzyme-linked immunosorbent assay(ELISA).The survival rates of neonates of the susceptible strain(ACB-BtS),Cry1Ab-resistant strain(ACB-AbR)and Cry1Ac-resistant strain(ACB-AcR)of the Asian corn borer(ACB),Ostrinia furnacalis,fed on whorl leaves of the transgenic maize ZZM030 and non-transgenic maize X249 were determined by using laboratory bioassays.【Results】The expression levels of Cry1Ab/Cry1Ac fusion protein in the whorl leaves of the transgenic maize ZZM030 at the 4-leaf stage and 8-leaf stage were 10.62 and 2.94μg/g FW,respectively.The survival rates of ACB-BtS neonates fed on whorl leaves of the transgenic maize ZZM030 for 2 d and more than 4 d were only 23.6%and 0,respectively,while the survival rate of ACB-BtS neonates fed on the non-Bt control maize X249 for 4 d was 93.1%.The survival rates of ACB-AbR and ACB-AcR neonates fed on whorl leaves of the transgenic maize ZZM030 for 6 d were 11.1%and 12.5%,respectively,whereas those fed on whorl leaves of non-Bt maize X249 for 6 d were 81.9%and 77.8%,respectively.【Conclusion】The highly expressed Cry1Ab/Cry1Ac fusion protein in transgenic cry1Ab/cry1Ac maize ZZM030 has an excellent control efficacy against ACB neonates.

关 键 词：亚洲玉米螟 转cry1Ab/cry1Ac基因玉米 杀虫效果 蛋白表达量 室内生测 

分 类 号：Q965.9[生物学—昆虫学]