猴头菇菌丝体多糖的超声辅助提取工艺研究  

Ultrasound-assisted extraction for polysaccharides frommycelium of hericium erinaceus

作　　者：王锋 刘晓鹏 王应玲[3] 张宝翠 付蕾 向晓双 陈莉莉 姜宁 WANG Feng;LIU Xiao-Peng;WANG Ying-ling;ZHANG Bao-cui;FU Lei;XIANG Xiao-shuang;CHEN Li-li;JIANG Ning(School of Biological Science and Technology,Hubei Minzu University,Enshi 445000,China;Hubei Key Laboratory of Biological Resources Protection and Utilization,Enshi 445000,China;No.2 Geological Brigade of Geological Department of Hubei Province,Enshi 445000,China)

机构地区：[1]湖北民族大学生物科学与技术学院,湖北恩施445000 [2]生物资源保护与利用湖北省重点实验室,湖北恩施445000 [3]湖北省地质局第二地质大队,湖北恩施445000

出　　处：《化学研究与应用》2020年第11期1984-1990,共7页Chemical Research and Application

基　　金：湖北省技术创新专项项目(2016AKB058,2017ABA139,2017AKB077)资助;国家自然科学基金项目(81460573)资助;国家“十二五”科技支撑计划项目(2011BAI04B04-5)资助;2019湖北产业教授人才项目(2019029)资助。

摘　　要：超声辅助提取多糖较传统的水浴法有着省时、节约能源等优点。本研究选取固液比、超声时间、超声功率、提取次数四个因素,采用单因素结合正交实验优化了超声辅助提取猴头菇菌丝体多糖的工艺。结果表明:超声辅助法提取的最佳方法为固液比1∶15(g·mL^-1)、超声时间10 min、超声功率200 W、提取次数3次。在此条件下,猴头菇菌丝体多糖的平均得率达(4.12±0.07)%。该研究为猴头菇菌丝体多糖提取的工业化生产提供了实验依据。Ultrasound-assisted extraction of polysaccharides has the advantages of time saving and energy saving compared with the traditional water bath method.In this study,the orthogonal experiments based on single factor tests were used to optimize the process of ultrasonic-assisted extraction of polysaccharides from hericium erinaceus mycelium,selecting four factors including solid-liquid ratio,ultrasonic time,ultrasonic power,and extraction times.The results showed that the best method for ultrasonic assisted extraction was solid-liquid ratio 1∶15(g·mL^-1),ultrasonic time 10 min,ultrasonic power 200 W and extraction times 3 times.Under these conditions,the average yield of mycelium polysaccharides from hericium erinaceus reached(4.12±0.07)%.This study could provide an experimental basis for the industrialization of the extraction of polysaccharides from the mycelium of hericium erinaceus.

关 键 词：猴头菇菌丝体 多糖 超声辅助 提取 

分 类 号：O636[理学—高分子化学]