痛泻要方对肝郁脾虚型IBS-D大鼠肠道菌群的影响研究  

Effect of Tongxieyaofang on gut microbiota in rats with diarrhea-predominant irritable bowel syndrome with liver depression and spleen deficiency

作　　者：张建峰 陈婕[2] 赵双梅[2] 王振雪 李慧臻[2] 胡建功[2] 郭慧颖 ZHANG Jianfeng;CHEN Jie;ZHAO Shuangmei;WANG Zhenxue;LI Huizhen;HU Jiangong;GUO Huiying(Tianjin University of Traditional Chinese Medicine,Tianjin 300193,Chian;The Second Affiliated Hospital of Tianjin University of Traditional Chinese Medicine,Tianjin 300250,China)

机构地区：[1]天津中医药大学,天津300193 [2]天津中医药大学第二附属医院,天津300250

出　　处：《湖南中医杂志》2020年第10期154-159,187,共7页Hunan Journal of Traditional Chinese Medicine

基　　金：天津市卫生局科技项目(2017137)。

摘　　要：目的:观察痛泻要方对肝郁脾虚型肠易激综合征(IBS-D)大鼠肠道菌群的影响,基于"脑肠菌轴"角度探讨痛泻要方治疗IBS-D的作用机制。方法:将24只SD大鼠随机分为4组:即空白组(Normal G)、模型组(ModelG)、双歧杆菌三联活菌片组(Shuang G)、痛泻要方组(Tong G),每组各6只。通过夹尾结合番泻叶灌胃的方法制备肝郁脾虚型IBS-D大鼠模型,分别给予相应药物灌胃,Normal G以及ModelG予0.9%氯化钠注射液灌胃。采用16srDNA测序法分析IBS-D大鼠肠道菌群在治疗前后的变化。结果:在门水平上,Tong G较ModelG增加了厚壁菌门、拟杆菌门的种群丰度,有效降低了含较多致病菌的变形菌门丰度;在纲水平上,Tong G较ModelG增加了梭状芽胞杆菌纲、拟杆菌纲、杆菌纲的丰度,降低了丙型变形菌纲丰度;在目水平上,Tong G较ModelG增加了梭菌目、乳杆菌目、拟杆菌目的丰度,降低了黄色单胞菌目、肠杆菌目丰度;在属水平上,Tong G较ModelG增加了巨单胞菌属、劳特氏菌属种群丰度,有效降低了寡养单胞菌属丰度;韦恩图和花瓣图显示,Normal G菌群数量最多,ModelG最少,Tong G较ModelG增多且程度大于Shuang G,共有种群数量更趋近于Normal G;稀释曲线和等级聚类曲线结果均显示Tong G的肠道菌群丰度及均匀度更趋近Normal G;箱型图展示Tong G与Normal G多样性、离散程度、中位数更接近;PCOA分析结果显示Tong G相较Shuang G,与Normal G重合度更高。结论:痛泻要方能纠正IBS-D患者存在的菌群失衡,促使肠道菌群结构趋于正常,且改善程度优于双歧杆菌三联活菌片。Objective: To investigate the effect of Tongxieyaofang on gut microbiota in rats with diarrheapredominant irritable bowel syndrome(IBS-D) with liver depression and spleen deficiency and the mechanism of action of Tongxieyaofang in the treatment of IBS-D from the aspect of "brain-gut-microbiota axis".Methods: A total of 24 Sprague-Dawley rats were randomly divided into blank group(NormalG group),model group(ModelG group),live combined Bifidobacterium and Lactobacillus tablets group(ShuangG group),and Tongxieyaofang group(TongG group),with 6 rats in each group.The rats were treated with tail clipping combined with senna by gavage to establish a model of IBS-D with liver depression and spleen deficiency,and related drugs were given by gavage;the rats in the NormalG group and the ModelG group were given 0.9% sodium chloride injection by gavage.16 srDNA sequencing was used to analyze the change in gut microbiota after treatment in IBS-D rats.Results: At the phylum level,compared with the ModelG group,the TongG group had significant increases in the abundance of Firmicutes and Bacteroidetes and a significant reduction in the abundance of Proteobacteria which contained many pathogens;at the class level,compared with the ModelG group,the TongG group had significant increases in the abundance of Clostridia,Bacteroidia,and Bacilli and a significant reduction in the abundance of Gammaproteobacteria;at the order level;compared with the ModelG group,the TongG group had significant increases in the abundance of Clostridiales,Lactobacillales,and Bacteroidales and significant reductions in the abundance of Xanthomonadales and Enterobacterales;at the genus level,compared with the ModelG group,the TongG group had significant increases in the abundance of Megamonas and Lauterella and a significant reduction in the abundance of Stenotrophomonas.Venn diagram and flower plot showed that the NormalG group had the highest number of flora and the ModelG group had the lowest number of flora,and the TongG group had a higher number than the

关 键 词：肠易激综合征 痛泻要方 脑肠菌轴 肠道菌群 实验研究 

分 类 号：R285.5[医药卫生—中药学]