双波长高效液相色谱法同时测定乌蕨中6种主要活性成分含量  

Simultaneous Determination of Six Active Components in Stenoloma chusanum(L.)Ching by Dual Wavelength HPLC

作　　者：陈明[1] 段萍 徐作刚 万红才 刘晓艳 CHEN Ming;DUAN Ping;XU Zuogang;WAN Hongcai;LIU Xiaoyan(Department of Pharmacology, School of Basic Mediccal Sciences,Qiannan Medical College for Nationalties, Duyun 558000, China;Chemistry Room, Qiannan Food and Drug Control Institute, Duyun 558000, China)

机构地区：[1]黔南民族医学高等专科学校基础医学部药理学教研室,都匀558000 [2]黔南州检验检测院食品药品检验所化学室,都匀558000

出　　处：《医药导报》2020年第12期1721-1725,共5页Herald of Medicine

摘　　要：目的建立同时测定乌蕨中原儿茶酸、原儿茶醛、荭草苷、牡荆素鼠李糖苷、牡荆素、木犀草苷等6种主要活性成分含量的双波长高效液相色谱法。方法选用赛默飞(Thermo)A型C18柱(4.6 mm×250 mm,5μm),以乙腈(A)-0.4%磷酸溶液(B)为流动相,梯度洗脱,原儿茶酸、原儿茶醛检测波长为280 nm,荭草苷、牡荆素鼠李糖苷、牡荆素、木犀草苷检测波长为350 nm。柱温:30℃,流速:1 mL·min-1;进样量:5μL。结果乌蕨中原儿茶酸、原儿茶醛、荭草苷、牡荆素鼠李糖苷、牡荆素、木犀草苷六种活性成分分离度良好,质量分别在0.1619~1.2144,0.0637~0.4779,0.0835~0.6265,0.2385~1.7885,0.0778~0.5837,0.0263~0.1970μg呈良好的线性关系,均r≥0.9999,平均回收率分别为100.42%,101.78%,99.17%,105.12%,97.86%,101.54%;RSD分别为2.38%,2.53%,2.31%,3.06%,2.13%,3.87%。结论该方法简单,结果准确,重复性、稳定性好,可为制订乌蕨的质量控制标准提供参考依据。Objective A dual wavelength high performance liquid chromatography(HPLC)method was established for the simultaneous determination of protocatechuic acid,protocatechuic aldehyde,orientin,oramnosylvitexin,vitexin,and luteolin-7-o-glucoside in Stenoloma chusanum(L.)Ching.Methods The analysis was performed on a Thermo-Finnegan C18(250 mm×4.6 mm,5μm)column with mobile phase of acetonitrile(A)-0.4%phosphoric acid solution(B)at the flow rate of 1.0 mL·min-1for gradient elution.The determintion wavelength was 280 nm for protocatechuic acid and protocatechui caldehyde,and 350 nm for orientin,oramnosylvitexin,vitexin and luteolin-7-o-glucoside.The column temperature was 30℃and injection volume was 5μL.Results The six compounds were well separated.The linear ranges were from 0.1619 to 1.2144,0.0637 to 0.4779,0.0835 to 0.6265,0.2385 to 1.7885,0.0778 to 0.5837,and 0.0263 to 0.1970μg for protocatechuic acid,protocatechuic aldehydeorientin,orientin,oramnosylvitexin,vitexin,luteolin-7-o-glucoside,respectively,with the correlation of r≥0.9999.The average recoveries were 100.42%(RSD 2.38%),101.78%(RSD 2.53%),99.17%(RSD 2.31%),105.12%(RSD 3.06%),97.86%(RSD 2.13%)and 101.54%(RSD 3.87%).Conclusion The method is simple,accurate,reproducible and stable,which can provide reference for the quality control standard of Stenoloma chusanum(L.)Ching.

关 键 词：乌蕨 原儿茶酸 原儿茶醛 荭草苷 牡荆素鼠李糖苷 牡荆素 木犀草苷 含量测定 色谱法 高效液相 

分 类 号：R286[医药卫生—中药学] R927.2[医药卫生—中医学]