GRM4基因在骨肉瘤细胞系MG-63细胞和Saos2细胞增殖和迁移中的作用  

The role of GRM4 gene in the proliferation and migration of MG-63 and Saos2 osteosarcoma cell lines

作　　者：张增亮 胡先同 张迎龙 赵彦涛[1] 李南 ZHANG Zeng-liang;HU Xian-tong;ZHANG Ying-long;ZHAO Yan-tao;LI Nan(Department of Orthopaedics,The Fourth Medical Center of PLA General Hospital,Beijing,100048,China)

机构地区：[1]解放军总医院第四医学中心骨科,北京100048

出　　处：《中国骨与关节杂志》2020年第11期869-872,共4页Chinese Journal of Bone and Joint

摘　　要：目的通过RNA干扰技术,敲低MG-63细胞和Saos2细胞中的GRM4基因,并对GRM4基因敲低后MG-63细胞和Saos2细胞的增殖及迁移能力进行研究。方法借助脂质体3000将GRM4的siRNA转染到MG-63细胞和Saos2细胞中,QPCR验证siRNA的干扰效果,通过CCK8法检测GRM4基因敲低后MG-63细胞和Saos2细胞的增殖情况,通过Transwell法检测GRM4基因敲低后MG-63细胞和Saos2细胞的迁移情况。结果siRNA成功转染到MG-63细胞中,干扰的效率为60%左右,转染到Saos2细胞中,干扰效率为61%左右;MG-63细胞和Saos2细胞的GRM4基因敲低后,细胞的增殖明显增加(MG-63 P=0.005;Saos2 P=0.003),并且细胞的迁移也明显增加(MG-63P=0.004;Saos2 P=0.006)。结论GRM4基因敲低后,MG-63细胞和Saos2细胞的增殖和迁移明显增强。Objective To knock down GRM4 gene in MG-63 cells and Saos2 cells by RNA interference technology,and to study the proliferation and migration ability of MG-63 cells and Saos2 cells after GRM4 gene knockdown.Methods GRM4 siRNA was transfected into MG-63 cells and Saos2 cells with the liposome 3000.QPCR was used to verify the interference effect of siRNA.Proliferation of MG-63 cells and Saos2 cells after GRM4 gene knockdown was detected by CCK8 method.Transwell method was used to detect the migration of MG-63 cells and Saos2 cells after GRM4 gene knockdown.Results The siRNA was successfully transfected into MG-63 cells with an interference efficiency of about 60%,while Saos2 cells of about 61%.Proliferation(MG-63 P=0.005;Saos2 P=0.003)and migration(MG-63 P=0.004;Saos2 P=0.006)were significantly increased after GRM4 gene knockdown.Conclusions Proliferation and migration of MG-63 cells and Saos2 cells are significantly enhanced after GRM4 gene knockdown.

关 键 词：骨肉瘤 GRM4 MG-63 Saos2 

分 类 号：R738.7[医药卫生—肿瘤]