雏鸡源金黄色葡萄球菌分离及生物学特性鉴定  

Isolation and Characterization of Staphylococcus aureus from Chickens

作　　者：张召兴[1,2] 李佩国 张海龙[1] 陈玥 李蕴玉 张香斋[1] 贾青辉[1] ZHANG Zhao-xing;LI Pei-guo;ZHANG Hai-long;CHEN Yue;LI Yun-yu;ZHANG Xiang-zhai;JIA Qing-hui(College of Animal Science and Technology,Hebei Normal University of Science and Technology,Heibei Province Key Laboratory of Veterinary Preventive Medicine,Qinhuangdao 066604,China;Hebei Tourism Vocational College Department of Animal Husbandry Medicine,Chengde 067000,China)

机构地区：[1]河北科技师范学院动物科技学院河北省预防兽医学重点实验室,河北秦皇岛066604 [2]河北旅游职业学院畜牧兽医系,河北承德067000

出　　处：《中国兽医杂志》2020年第7期81-84,I0011,共5页Chinese Journal of Veterinary Medicine

基　　金：河北省现代农业产业技术体系蛋鸡产业创新团队项目(HBCT2018150206)。

摘　　要：为了鉴定引起雏鸡关节炎及死亡的病原菌的生物学特性,采用常规细菌分离鉴定和16S rRNA PCR等方法从无菌采集病死雏鸡的肝脏、关节渗出液等病料组织中分离得到1株金黄色葡萄球菌。采用人工感染动物试验、K-B药敏纸片法及PCR法分别检测分离菌株的致病性、耐药性及多重耐药基因(cfr、fexA、fexB)携带情况。结果表明,分离菌株对雏鸡均有很强的致病性,其半数致死量(LD 50)为3.16×106 CFU/mL;分离菌株对头孢曲松、头孢拉啶、环丙沙星等5种药物敏感,对其他药物具有不同程度的耐药性;分离菌株同时携带多重耐药基因cfr和fexA。In order to identify the biological characteristics of pathogens causing arthritis and death in chickens,a strain of Staphylococcus aureus was isolated from liver and joint exudates of dead chickens by routine bacterial isolation and identification and 16S rRNA PCR.The pathogenicity,drug resistance and multiple drug resistance genes(cfr,fexA,fexB)were detected by artificial infection animal test,K-B susceptibility paper method and PCR method,respectively.The results showed that the isolates were highly pathogenic to chickens,with LD 50 of 3.16×106 CFU/mL.Isolated strains were sensitive to five drugs such as ceftriaxone,cefradine and ciprofloxacin,and were resistant to other drugs to varying degrees.The isolates also carried multiple drug resistance genes cfr and fexA.

关 键 词：雏鸡 金黄色葡萄球菌 生物学特性 多重耐药基因 

分 类 号：S831[农业科学—畜牧学]