Bioaugmentation and quorum sensing disruption as solutions to increase nitrate removal in sequencing batch reactors treating nitrate-rich wastewater  

作　　者：Xinyi Ruan Jun Yin Xueyan Cui Na Li Dongsheng Shen 

机构地区：[1]School of Environmental Science and Engineering,Zhejiang Gongshang University,Hangzhou 310012,PR China [2]Zhejiang Provincial Key Laboratory of Solid Waste Treatment and Recycling,Hangzhou 310012,PR China

出　　处：《Journal of Environmental Sciences》2020年第12期179-185,共7页环境科学学报（英文版）

基　　金：supported by the Natural Science Foundation of Zhejiang Province (nos. LY17E080001 and LQ18E080005);the China Scholarship Council (no. iCET 2017)。

摘　　要：Bioaugmentation of denitrifying bacteria can serve as a promising technique to improve nutrient removal during wastewater treatment. While denitrification inhibition by bacterial quorum sensing(QS) in Pseudomonas aeruginosa has been indicated, the application of bacterial QS disruption to improve nitrate removal from wastewater has not been investigated. In this study, the effect of bioaugmentation of P. aeruginosa SD-1 on nitrate removal in sequencing batch reactors that treat nitrate rich wastewater was assessed. Additionally, the potential of a quorum sensing inhibitor(QSI) to improve denitrification following bacterial bioaugmentation was evaluated. Curcumin, a natural plant extract, was used as a QSI. The chemical oxygen demand(COD) and initial nitrate concentration of the influent were 700 ±20 mg/L and 200 ±10 mg/L respectively, and their respective concentrations in the effluent were 56.9 ±3.2 mg/L and 9.0 ±3.2 mg/L. Thus, the results revealed that bioaugmentation of P. aeruginosa SD-1 resulted in an increased nitrate removal to 82% ±1%. Further, nitrate was almost completely removed following the addition of the QSI, and activities of nitrate reductase and nitrite reductase increased by 88% ±2% and 74% ±2% respectively. The nitrogen mass balance indicated that aerobic denitrification was employed as the main pathway for nitrogen removal in the reactors. The results imply that bioaugmentation and modulation of QS in denitrifying bacteria, through the use of a QSI, can enhance nitrate removal during wastewater treatment.

关 键 词：BIOAUGMENTATION DENITRIFICATION Nitrate removal Quorum sensing inhibitor CURCUMIN 

分 类 号：X703[环境科学与工程—环境工程]