Development of peanut varieties with high oil content by in vitro mutagenesis and screening  

作　　者：WANG Jing-shan SHI Lei LIU Yue ZHAO Ming-xia WANG Xia QIAO Li-xian SUI Jiong-ming LI Guan ZHU Hong YU Shan-lin 

机构地区：[1]College of Agronomy,Qingdao Agricultural University/Shandong Provincial Key Laboratory of Dryland Farming Technology,Qingdao 266109,P.R.China [2]Haidu College,Qingdao Agricultural University,Laiyang 265200,P.R.China [3]College of Horticulture,Qingdao Agricultural University,Qingdao 266109,P.R.China

出　　处：《Journal of Integrative Agriculture》2020年第12期2974-2982,共9页农业科学学报（英文版）

基　　金：This research was funded by the National Natural Science Foundation of China(31571705 and 31872875);the Key R&D Program of Shandong Province,China(2018GNC111014);the Open Oroject of the Key Lab of Oil Crop Biology and Genetic Breeding,Ministry of Agriculture and Rural Affairs of China(KF2018008).

摘　　要：Peanut (Arachis hypogaea L.) is an important oil crop globally and high oil content is one of the major targets in peanut breeding programs. Previous studies indicated that the osmotic pressure (OP) of the leaves of peanut plants subjected to drought stress was negatively correlated with kernel oil content. Based on this knowledge, we established a practical and reliable method for creating new peanut varieties with high oil content using in vitro mutagenesis and directional OP-based selection. Using embryonic leaflets of peanut variety Huayu 20 as explants, pingyangmycin (PYM) as the mutagen, and hydroxyproline (HYP) as the OP regulator, we developed 15 HYP-tolerant regenerated plants. For each regenerated plant, we selected offspring with oil content>55% (relative to 49.5% for Huayu 20). We developed and released three new peanut varieties with high yield and high oil content from the offspring of the HYP-tolerant regenerated plants. The three new varieties were named as Yuhua 4, Yuhua 9 and Yuhua 14 and their oil contents were 57.7, 61.1 and 59.3%, respectively. The results indicate that in vitro mutagenesis with PYM followed by directed screening with HYP is a useful approach for breeding peanut varieties with high oil contents.

关 键 词：Arachis hypogaea in vitro mutagenesis HYP-directed selection high oil variety 

分 类 号：S565.2[农业科学—作物学]