The Chromosome-Level Genome Sequence of the Autotetraploid Alfalfa and Resequencing of Core Germplasms Provide Genomic Resources for Alfalfa Research  

作　　者：Chen Shen Huilong Du Zhuo Chen Hongwei Lu Fugui Zhu Hong Chen Xiangzhao Meng Qianwen Liu Peng Liu Lihua Zheng Xiuxiu Li Jiangli Dong Chengzhi Liang Tao Wang 

机构地区：[1]State Key Laboratory of Agrobiotechnology,College of Biological Sciences,China Agricultural University,Beijing,China [2]State Key Laboratory of Plant Genomics,Institute of Genetics and Developmental Biology,Innovation Academy for Seed Design,Chinese Academy of Sciences,Beijing,China [3]University of Chinese Academy of Sciences,Beijing,China

出　　处：《Molecular Plant》2020年第9期1250-1261,共12页分子植物（英文版）

基　　金：supported by the National Key Research&Development Program of China(2019YFD1002701);Agricultural Variety Improvemnt Project of Shandong Province(2019LZGC010);the Project for Extramural Scientists of the State Key Laboratory for Agrobiotech no logy(2020SKLAB6-15,2011SKLAB01-1).

摘　　要：Alfalfa(Medicago sativa)is one of the most important forage crops in the world;however,its molecular genetics and breeding research are hindered due to the lack of a high-quality reference genome.Here,we report a de novo assembled 816-Mb high-quality,chromosome-level haploid genome sequence for‘Zhongmu No.1’alfalfa,a heterozygous autotetraploid.The contig N50 is 3.92 Mb,and 49165 genes are annotated in the genome.The alfalfa genome is estimated to have diverged from M.truncatula approximately 8 million years ago.Genomic population analysis of 162 alfalfa accessions revealed high genetic diversity,weak population structure,and extensive gene flow from wild to cultivated alfalfa.Genome-wide association studies identified many candidate genes associated with important agronomic traits.Furthermore,we showed that MsFTa2,a Flowering Locus T homolog,whose expression is upregulated in salt-resistant germplasms,may be associated with fall dormancy and salt resistance.Taken together,these genomic resources will facilitate alfalfa genetic research and agronomic improvement.

关 键 词：ALFALFA genome assembly population gen etics GW AS Flowering Locus T 

分 类 号：Q943.2[生物学—植物学]