高效液相色谱法测定邻苯二甲醛中的有机杂质  

Determination of organic impurities in ortho-phthalaldehyde by high performance liquid chromatography

作　　者：吴素芳[1] 成西涛[1] 沈寒晰[1] 黄方方[1] 白燕[1] Wu Su-fang;Cheng Xi-tao;Shen Han-xi;Huang Fang-fang;Bai Yan(Shaanxi Research Design Institute of Petroleum and Chemical Industry,Shaanxi Key Laboratory of petroleum Fine Chemicals,Xi’an,Shaanxi 710054,China)

机构地区：[1]陕西省石油化工研究设计院,陕西省石油精细化学品重点实验室,陕西西安710054

出　　处：《福建分析测试》2020年第5期39-41,45,共4页Fujian Analysis & Testing

摘　　要：建立高效液相色谱法同时测定邻苯二甲醛中有机杂质邻苯二甲酸、2-羧基苯甲醛和苯酞等含量的分析方法。采用Agilent ZORBAX SB-AQ色谱柱(150mm×4.6mm,5μm),流动相为乙腈-0.1%甲酸水溶液,梯度洗脱;流速1.0mL/min;检测波长254nm;柱温25℃。实验结果表明,3种物质在质量浓度为0.5~100μg/mL时呈良好的线性关系,相关系数r均大于0.9995,回收率在99%~101%之间,相对标准偏差(n=5)小于2.047%。该方法准确、高效,可用于邻苯二甲醛产品的杂质分析。A HPLC method was developed for the determination of phthalic acid,2-carboxybenzaldehyde and phthalide in ortho-phthalaldehyde.HPLC was used with agilent ZORBAX SB-AQ chromatography column(150mm×4.6mm,5μm)as the column and acetonitrile-0.1%formic acid aqueous solution.A gradient elution program at a flow rate of 1.0mL/min was utilized for the separation and determination.The detection wavelength was set at 254nm,and the column temperature was 25℃.The results showed that the calibration curves for them was linear in the range of 0.5~100μg/mL and the correlation coefficients were more than 0.9995.The recoveries were 99%~101%,RSD(n=5)were less than 2.047%.This method is accurate,efficient and can be used for the determination of ortho-phthalaldehyde.

关 键 词：高效液相色谱法 邻苯二甲醛 邻苯二甲酸 2-羧基苯甲醛 苯酞 

分 类 号：O657.72[理学—分析化学]