保幼激素诱导尖唇散白蚁兵蚁分化的特征及其相关基因的表达谱  

Characteristics of soldier differentiation of termite Reticulitermes aculabialis induced by juvenile hormone and expression profiling of juvenile hormone-related genes

作　　者：楚君鹏 伍志伟 杜运良 席玉强[2] 安世恒[2] 苏丽娟[1] CHU Junpeng;WU Zhiwei;DU Yunliang;XI Yuqiang;AN Shiheng;SU Lijuan(College of Life Sciences,Henan Agricultural University,Zhengzhou 450002,Henan Province,China;College of Plant Protection,Henan Agricultural University,Zhengzhou 450002,Henan Province,China)

机构地区：[1]河南农业大学生命科学学院,郑州450002 [2]河南农业大学植物保护学院,郑州450002

出　　处：《植物保护学报》2020年第5期1099-1107,共9页Journal of Plant Protection

基　　金：河南省科学技术厅基础与前沿技术研究(142300410150);河南省高等学校重点科研项目(20A210004)。

摘　　要：为明确保幼激素调控白蚁兵蚁分化的机理,以尖唇散白蚁Reticulitermes aculabialis为研究对象,用保幼激素Ⅲ及其类似物吡丙醚诱导其工蚁向兵蚁分化,并用荧光定量PCR技术对兵蚁分化过程中保幼激素信号相关基因的表达模式进行分析。结果显示,春、夏季节保幼激素Ⅲ和吡丙醚对尖唇散白蚁前兵蚁的诱导率和死亡率均高于秋、冬季节,如在春、夏季的诱导率最高达60.84%,死亡率最高为47.18%;而在秋、冬季的诱导率最高仅为20.00%,死亡率最高为12.50%。相同浓度的保幼激素Ⅲ和吡丙醚诱导尖唇散白蚁时,在前兵蚁阶段Met、Kr-h1、Br-C和USP基因的表达模式基本相似,除了USP外,均有表达高峰;Met在前兵蚁阶段的第5天达到高峰;保幼激素Ⅲ诱导与吡丙醚诱导相比,Kr-h1和Br-C的表达高峰均提前2 d。不同浓度的吡丙醚诱导白蚁时,120μg/mL和160μg/mL浓度诱导下Met、Kr-h1和Br-C基因均在第5天出现表达高峰(Br-C的160μg/mL处理除外,表达高峰推迟2 d)。表明春、夏季保幼激素及其类似物更容易诱导分化出前兵蚁,且保幼激素Ⅲ比吡丙醚对下游基因的影响更明显。In order to explore the mechanism of termite soldier differentiation regulated by juvenile hormone, Reticulitermes aculabialis was used to investigate the differentiation of worker-to-soldier by using juvenile hormone Ⅲ and its analogue-pyripropoxyfen, and expression profiling of juvenile hormone signal-related genes was analyzed using real-time fluorescence quantitative PCR during the pre-soldier phase. The results showed that the inductivity and mortality of pre-soldier by juvenile hormone Ⅲ and pyripropoxyfen in spring and summer were higher than that in autumn and winter: the highest inductivity and mortality were 60.84% and 47.18%, respectively, in spring and summer, while those were only20.00% and 12.50%, respectively, in autumn and winter. The expression patterns of the genes Met, Krh1, Br-C and USP were similar, except for USP, when the pre-soldiers were induced with the same concentration of juvenile hormone Ⅲ and pyripropoxyfen. The peak of Met was observed on the 5 th day of pre-soldier;in contrast, the expression peaks of Kr-h1 and Br-C appeared two days earlier. With pyripropoxyfen inducing workers at different concentrations, the expression peaks of Met, Kr-h1 and Br-C were observed at the 5 th day of pre-soldier in 120 μg/mL and 160 μg/mL groups(except for Br-C in 160μg/mL group, which was delayed for two days). These results indicated that juvenile hormone and its analogue induced pre-worker more easily in spring/summer than in autumn/winter, and juvenile hormone Ⅲ imposed a more obvious impact on downstream genes than that of pyripropoxyfen.

关 键 词：保幼激素 吡丙醚 尖唇散白蚁 诱导 兵蚁等级分化 

分 类 号：S476[农业科学—农业昆虫与害虫防治]