小麦芒抑制基因Hd的定位和候选基因分析  

Mapping and Candidate Gene Analysis of Awn Inhibition Gene Hd in Wheat(Triticum aestivum)

作　　者：杨思晴 胡乐佳 胡鑫 戎均康[2] YANG Si-Qing;HU Le-Jia;HU Xin;RONG Jun-Kang(College of Forestry and Biotechnology,Zhejiang A&F University,Lin'an 311300,China;College of Agriculture and Food Science,Zhejiang A&F University,Lin'an 311300,China)

机构地区：[1]浙江农林大学林业与生物技术学院,临安311300 [2]浙江农林大学农业与食品科学学院,临安311300

出　　处：《农业生物技术学报》2020年第11期1901-1912,共12页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金(31671684);浙江省青年自然科学基金(LQ20C130001)。

摘　　要：小麦(Triticum aestivum)麦芒在抵抗虫害、种子繁殖、增加小麦产量等方面具有重要的作用,是小麦重要的农艺性状,受多个基因控制。CASL4AL是以普通小麦品种’中国春’(T.aestivum cv.Chinese Spring,CS,帽状的钩芒)为背景的野生二粒小麦(T.dicoccoides,TTD)TTD140染色体臂置换系(chromosome arm substitution line,CASL),其中4A染色体长臂被野生二粒小麦TTD140所置换,表现为长芒。本研究利用长芒的染色体臂置换系CASL4AL与帽状钩芒的442株系(CASL4AL和CS杂交后代,其与CS的芒表型一致)构建1个F2群体(134个单株),采用SSR分子标记,利用完备区间作图(inclusive composite interval mapping,ICIM)法对小麦芒长进行QTL定位。检测到1个位于4AS染色体与芒长相关的QTL,LOD值30.71,解释82.63%的表型变异。在该定位区间,对CASL4AL重测序数据相对参考基因组的变异(SNP和InDel)进行注释,并结合转录组测序差异分析,共获得了5个差异表达且存在有义变异的基因,其中TraesCS4A02G078700翻译MADS-box转录因子(MADS-box transcription factor 1),参与芒外稃和内稃分生组织的分化和增殖,可能参与调控麦芒的生长。本研究为精细定位材料的筛选和麦芒抑制基因Hd(Hooded)的精细定位提供了依据。Wheat(Triticum aestivum)awn,an important agronomic character of wheat controlled by many genes,plays an important role in resisting insect pests,seed reproduction and increasing wheat yield.CASL4AL is a chromosome arm substitution line(CASL)of wild emmer(T.dicoccoides,TTD)accession TTD140 in the genetic background of common wheat variety Chinese Spring(T.aestivum cv.Chinese Spring,CS),the long arm of chromosome 4A of CASL4AL is replaced by TTD140,showing long awn.In this study,a F2 population with 134 lines was constructed by using CASL4AL with long awn and the line of 442 with hook awn(derived from the cross of CASL4AL×CS,hooded awn as CS).The SSR molecular markers were used for QTL mapping for the awn length of wheat by ICIM(inclusive composite interval mapping)method.A QTL with LOD value of 30.71 was detected on the chromosome 4AS related to awn length,explaining 82.63% of the phenotypic variation.In this mapping interval,the annotation of the variants(SNP and InDel)for the CASL4AL resequencing data were conducted according to reference wheat genome,combined with the differential analysis of transcriptome sequencing,five differentially expressed genes with sense variation were obtained,among which,TraesCS4 A02G078700 translates MADS-box transcription factor 1,which involved in the differentiation and proliferation of the meristem of lemma and palea,and probably involved in regulating the growth of wheat awn.This study provide reference for the screening of fine mapping materials and fine mapping of awn gene Hd(Hooded).

关 键 词：染色体臂置换系 芒 钩芒基因 QTL定位 重测序 转录组 

分 类 号：S512.1[农业科学—作物学]