马尾松苗期转录组bHLH基因家族成员鉴定及表达分析  

Identification and Expression Analyses of bHLH Gene Family Members in Transcriptome of Pinus massoniana Seedlings

作　　者：尚先文 范付华[1,2] 周紫晶 覃慧娟 王聪慧 丁贵杰[1,2] 谭健晖[3] SHANG Xian-Wen;FAN Fu-Hua;ZHOU Zi-Jing;QIN Hui-Juan;WANG Cong-Hui;DING Gui-Jie;TAN Jian-Hui(Institute for Forestry Resources&Environment of Guizhou/Key Laboratory of Forest Cultivation in Plateau Mountain of Guizhou,Guizhou University,Guiyang 550025,China;College of Forestry,Guizhou University,Guiyang 550025,China;Guangxi Zhuang Autonomous Region Forestry Research Institue,Nanning 530002)

机构地区：[1]贵州大学贵州省森林与环境研究中心/高原山地林木培育重点实验室,贵阳550025 [2]贵州大学林学院,贵阳550025 [3]广西壮族自治区林业科学研究院,南宁530002

出　　处：《农业生物技术学报》2020年第11期1947-1959,共13页Journal of Agricultural Biotechnology

基　　金：国家重点研发计划(2017YFD0600302);国家自然科学基金(31660185);广西创新驱动发展专项(桂科AA17204087-4);贵州省一流学科建设项目(GNYL[2017]007)。

摘　　要：植物碱性螺旋-环-螺旋(basic helix-loop-helix,bHLH)转录因子是真核生物体内第二大类转录因子,在植物的生长发育、次生代谢和逆境响应中起着重要作用。本研究对马尾松(Pinus massoniana)苗期根样品进行转录组测序,筛选出bHLH家族基因序列,运用生物信息学方法对其进行亚细胞定位、保守基序(motif)、表达模式及系统进化等分析。结果显示,从马尾松转录组中共鉴定得到64个马尾松bHLH家族基因,经预测主要定位在细胞核;保守基序中motif1和motif2出现频率最多;在低磷胁迫条件下,PmbHLH01/09/27/29/32/39/41等基因呈显著差异表达,可能参与低磷胁迫响应。本研究结果为进一步揭示马尾松bHLH转录因子响应磷素改变的生物学功能提供参考依据。Plant basic helix-loop-helix(bHLH)transcription factors are the second largest class of transcription factors in eukaryotes,and play important roles in plant growth,secondary metabolism and stress response.In this study,bHLH family genes were screened by transcriptome sequencing of root samples of Pinus massoniana seedlings.The subcellular location,conservative motifs,expression pattern and phylogenetic analyses of these genes were performed by bioinformatics methods.The results showed that 64 bHLH family genes were identified from the transcriptome of Pinus massoniana,and these genes were predicted to be mainly located in the nucleus.Motif1 and motif2 appeared most frequently in conserved motifs.Under the condition of lowphosphorus stress,PmbHLH01/09/27/29/32/39/41 showed significantly differential expression,suggesting that they might be involved in phosphorus stress.These results provide a reference for further revealing the biological function of b HLH transcription factors in response to phosphorus change of P.massoniana.

关 键 词：马尾松 碱性螺旋-环-螺旋(bHLH)基因家族 低磷胁迫 表达分析 

分 类 号：S722.36[农业科学—林木遗传育种] Q943[农业科学—林学]