头颈部鳞癌中微RNA-202靶基因的预测及生物信息学方法分析  

Prediction and bioinformatics analysis of target genes of miR-202 in head and neck squamous cell carcinoma

作　　者：韩向春 郑力强 李燕妮[3] 黄勇 傅英 丁建花 孙娜 李广宁 Han Xiangchun;Zheng Liqiang;Li Yanni;Huang Yong;Fu Ying;Ding Jianhua;Sun Na;Li Guangning(Department of Pathology,Tianjin Beichen Traditional Chinese Medicine Hospital,Tianjin 300400,China)

机构地区：[1]天津市北辰区中医医院病理科,300400 [2]解放军陆军第81集团军医院皮肤科 [3]天津市口腔医院中心实验室

出　　处：《山西医药杂志》2020年第21期2912-2915,I0001,共5页Shanxi Medical Journal

摘　　要：目的采用生物信息学的方法预测头颈部鳞癌中微RNA(miR)-202的靶基因及相关功能,为后续研究提供理论基础。方法使用BLAST分析miR-202序列保守性;下载GEO相关数据,构建头颈部鳞癌miRNA表达谱;利用miRanda、TargetScan7.2、miRDB和miRTarBase预测结果取交集为预测靶基因,进一步做GO分析和Pathway分析以及miRNA-mRNA网络调控图。结果miR-202在多种物种间具有高度保守性,共获得43个靶基因,功能富集分析主要集中经典PID AP1通路、间质发展、组织形态发生和调节蛋白质的丝氨酸/苏氨酸激酶活性等生物学过程。miRNA-mRNA调控网络示miR-202与靶基因基质金属蛋白酶1(MMP1)、湿疹血小板减少伴免疫缺陷综合征样蛋白(WASL)、转化生长因子-β-Ⅲ型受体(TGFβ-R3)和白细胞介素6受体(IL6R)存在密切调控关系。结论miR-202在头颈部鳞癌中可通过与MMP1、WASL、TGFβ-R3和IL6R结合发挥调控作用。Objective To predict the target genes and related functions of miR-202 in head and neck squamous cell carcinoma(SCC)by bioinformatics method,so as to provide theoretical basis for subsequent studies.Methods The conservatism of miR-202 sequences was analyzed by BLAST.GEO related data were downloaded to construct the microRNA expression profile of HNSCC.The intersection of prediction results of miRanda,TargetScan7.2,miRDB and miRTarBase were taken as the predicted target genes,and GO analysis,pathway analysis and miRNA-mRNA network regulation map were further conducted.Results miR-202 was highly conserved among various species,and a total of 43 target genes were obtained.Functional enrichment analysis focused on biological processes such as classical PID AP1 pathway,mesenchyme development,tissue morphogenesis and regulation of serine/threonine kinase activity of proteins.The miRNA-mRNA regulatory network showed that miR-202 was closely regulated by the target genes MMP1,WASL,TGFβ-R3 and IL6R.Conclusion miR-202 plays a regulatory role in HNSCC by combining with MMP1,WASL,TGFβ-R3 and IL6R.

关 键 词：微RNAS 基因 计算生物学 肿瘤 

分 类 号：R739.91[医药卫生—肿瘤]