运动血清对海马HT-22细胞突触形成及BDNF、TrkB蛋白表达的影响  

Effects of exercise serum on synapse formation and expression of Bdnf and Trkb in hippocampal HT-22 cells

作　　者：魏宁[1] 季师敏[2] 刘钢[2] 胡斐 魏翠 黄夏 王海亚 郭世磊 王斌[4] WEI Ning;JI Shimin;LIU Gang;HU Fei;WEI Cui;HUANG Xia;WANG Haiya;GUO Shilei;WANG Bin(Graduate Department of Nanjing Sport Institute,Nanjing 210014,Jiangsu,China;Jiangsu Institute of Sports Science,Nanjing 210033,Jiangsu,China;Nanjing Regenecere Bin Limited Conipany,Nanjing 214000,Jiangsu,China;Sports and Health Collepn of Nanjing Sport Astitute,Nanjing 214014,Jiangsu,China)

机构地区：[1]南京体育学院研究生部,江苏南京210014 [2]江苏省体育科学研究所,江苏南京210033 [3]南京瑞吉科生物科技有限公司,江苏南京210000 [4]南京体育学院运动与健康学院,江苏南京210014

出　　处：《辽宁体育科技》2020年第6期52-56,共5页Liaoning Sport Science and Technology

基　　金：江苏省高校自然科学研究重大项目“基于可穿戴设备的运动应激水平评估与学习记忆机能”(项目编号:15KJA180005);2019年江苏省研究生培养创新工程研究生科研与实践创新计划研究成果(项目编号:KYCX19_1489)。

摘　　要：目的:探讨运动血清对海马HT-22细胞突触形成和BDNF、TrkB蛋白表达量的影响。方法:测定运动员和无运动习惯人血清睾酮、皮质醇、肌酸激酶和尿素含量,分别采用运动员血清、无运动习惯人血清干预离体培养的海马HT-22细胞,观察不同血清培养下海马HT-22细胞的形态,并测定海马HT-22细胞BDNF和TrkB两种蛋白的表达量。结果:在进行运动训练后,血清中生化指标有了一定的变化;运动血清培养的HT-22细胞间的连接数量多于无运动习惯人;男性运动血清组细胞BDNF和TrkB含量均显著大于无运动习惯男性血清组(P<0.05);女性运动血清组细胞TrkB含量显著大于无运动习惯型女性血清组(P<0.05)。结论:运动血清能够增加HT-22细胞间的连接数量以及BDNF、TrkB的表达,对海马产生有益的影响。Objective:To investigate the effect of exercise serum on synapse formation and expression of BDNF and TrkB in HT-22 cells.Methods:The biochemical indexes of serum of athletes and people without exercise habit,including testosterone,cortisol,creatine kinase and urea nitrogen,were measured.The HT-22 cells in vitro were treated with the serum of athletes and people without exercise habits.The morphology of HT-22 cells in different serum cultures was observed,and the expression of BDNF and TrkB in HT-22 cells were measured.Results:After the exercise training,the biochemical indexes in serum had certain changes;The number of connections between HT-22 cells cultured with exercise serum was more than that of exercise habit people;The content of BDNF and TrkB in the male exercise serum group was significantly higher than that in non-exercise habit type male serum group(P<0.05);The content of TrkB in female exercise serum group was significantly higher than that in non-exercise habit type female serum group(P<0.05)(<0.05).Conclusion:Exercise serum can increase the number of connections between HT-22 cells and the expression of BDNF and TrkB,which has a beneficial effect on the hippocampus.

关 键 词：海马HT-22细胞突触 BDNF TRKB 蛋白表达 

分 类 号：G802.0[文化科学—运动人体科学]