过量表达EPSPS转基因对栽培稻与杂草稻杂种后代稻瘟病抗病性的影响  

Effect of overexpression of EPSPS gene on blast resistance of hybrid offspring of cultivated rice and weedy rice

作　　者：林凯青 韩光煜 罗琼 何平 南蓬[1] LIN Kaiqing;HAN Guangyu;LUO Qiong;HE Ping;NAN Peng(School of Life Sciences,Fudan University,Key Laboratory of Biodiversity Sciences and Ecological Engineering,Shanghai 200438,China;College of Plant Protection,Yunnan Agricultural University,Key Laboratory of Agrobiodiversity and Pest Management,Ministry of Education,Kunming 650201,China)

机构地区：[1]复旦大学生命科学学院,生物多样性与生态工程教育部重点实验室,上海200438 [2]云南农业大学植物保护学院,农业生物多样性与病害控制教育部重点实验室,昆明650201

出　　处：《植物生理学报》2020年第9期1872-1880,共9页Plant Physiology Journal

基　　金：国家转基因生物新品种培育科技重大专项(2016ZX08011006)。

摘　　要：5-烯醇式丙酮酰莽草酸-3-磷酸合酶(5-enolpyruvylshikimate-3-phosphate synthase,EPSPS)是植物体莽草酸途径中除草剂草甘膦的靶标酶。本研究利用过量表达EPSPS转基因栽培稻×杂草稻杂种后代(WH1)进行转录组测序研究,发现水杨酸信号转导途径发生显著富集;稻瘟病抗病性鉴定实验结果表明,过量表达EPSPS转基因栽培稻×杂草稻杂种阳性后代(WH1+)与阴性后代(WH1–)的稻瘟病抗病性没有显著差异;代谢物含量检测表明,转基因阳性植株与阴性植株的水杨酸含量基本一致。由此可见,过量表达EPSPS转基因事件不会通过改变水杨酸水平来影响栽培稻×杂草稻杂种后代植株的稻瘟病抗病性。5-Enolpyruvylshikimate-3-synthase(EPSPS)is the target enzyme for herbicide glyphosate in the shikimate pathway.In this study,RNA sequencing was carried out and we found that,in the descendants of overexpressing EPSPS transgenic rice line×weedy rice hybrid offspring(WH1),the salicylic acid signal pathway was significantly enriched.With rice blast resistance test,we found that there was no significant difference in resistance to rice blast between positive offspring(WH1+)and negative offspring(WH1–),and the salicylic acid contents of WH1+and WH1–had no significant difference.It can be concluded that the overexpressing of EPSPS transgene will not affect the rice blast resistance of the offsprings from transgenic line and weedy rice hybrid by changing the content of salicylic acid.

关 键 词：EPSPS 稻瘟病抗病性 转录组 水杨酸 LC-MS 

分 类 号：S511[农业科学—作物学]