内生真菌侵染对化感物质胁迫下木麻黄幼苗根系活性氧代谢及保护酶系统的影响  

Effects of endophyte infection on reactive oxygen metabolism and protective enzyme system of Casuarina equisetifolia seedlings under allelopathic stress

作　　者：刘鑫铭 郭汝凤 刘奕[1] 林勇明[1] 吴承祯 李键[1] LIU Xinming;GUO Rufeng;LIU Yi;LIN Yongming;WU Chengzhen;LI Jian(College of Forestry,Fujian Agriculture and Forestry University,Fuzhou 350002,China;College of Ecology and Resource Engineering,Wuyi University,Nanping,Fujian 354300,China;Key Laboratory for Eco-Industrial Green Technology of Fujian Province,Wuyi University,Nanping,Fujian 354300,China)

机构地区：[1]福建农林大学林学院,福州350002 [2]武夷学院生态与资源工程学院,福建南平354300 [3]武夷学院福建省生态产业绿色技术重点实验室,福建南平354300

出　　处：《植物生理学报》2020年第9期1985-2006,共22页Plant Physiology Journal

基　　金：国家自然科学基金(31400533和32071753);福建省自然科学基金(2014J01074);福建农林大学科技创新专项基金项目(CXZX2016052和CXZX2018122);福建农林大学林学高峰学科建设项目(71201800705)。

摘　　要：本文对未侵染(EF)和侵染(EI)内生真菌(Phyllosticta sp.)的木麻黄幼苗分别进行5种提取自木麻黄根系的化感物质[12,13-dihydromicromeric acid、白桦脂酸、3-O-咖啡酰基羽扇豆醇、儿茶素和表儿茶素]胁迫,比较不同胁迫浓度和胁迫时间下内生真菌对木麻黄幼苗根系活性氧代谢以及保护酶系统的影响。结果表明:化感胁迫浓度、胁迫时间和内生真菌对木麻黄幼苗根系活性氧代谢(O^-·2产生速率、H2O2和MDA含量)和保护酶系统(CAT、POD和SOD活性)有交互作用。化感胁迫导致O^-·2产生速率、H2O2和MDA含量显著升高,CAT、POD和SOD活性显著升高后降低,达到峰值的时间提前。在较低浓度(12.5、25、50和100 mg·L-1)和较短时间(0~48 h)胁迫下内生真菌能够显著提高保护酶活性并抑制活性氧含量的增加(P<0.05)。随胁迫浓度和时间增加,内生真菌对木麻黄保护酶活性的促进作用受到抑制。化感物质种类影响木麻黄幼苗根系受伤害的程度,较低浓度(0~50 mg·L-1)和较短时间(0~36 h)胁迫下3-O-咖啡酰基羽扇豆醇对木麻黄伤害程度最大,儿茶素伤害程度最小;较高浓度(100~400 mg·L-1)和较长时间(48~72 h)胁迫下12,13-dihydromicromeric acid对木麻黄伤害程度最大,白桦脂酸伤害程度最小。In this paper,five kinds of allelochemicals(12,13-dihydromicromeric acid,betulinic acid,3-O-coffee-acyl-lupeol,catechin and epicatechin)extracted from the roots of Casuarina equisetifolia were applied to C.equisetifolia seedlings of uninfected(EF)and infected(EI)endophyte(Phyllosticta sp.)and the effects of different stress concentrations and stress time on active oxygen metabolism and protective enzyme system in roots were compared.The results showed that there were interactions of allelopathic stress concentration,stress time and endophyte with active oxygen metabolism(O^-·2production rate,H2 O2 and MDA contents)and protective enzyme system(CAT,POD and SOD activities)of C.equisetifolia roots.Allelopathic stress led to a significant increase in O2-·production rate,H2 O2 and MDA contents(P<0.05),and a significant decrease after increase in CAT,POD and SOD activities(P<0.05),and reached the peak time earlier.Endophyte with low concentration(12.5,25,50,100 mg·L-1)and short time(0–48 h)stress could significantly increase the activities of protective enzymes and inhibit the increase of active oxygen content(P<0.05).With the increase of stress concentration and time,the promoting effect of endophyte on the protective enzyme activities of C.equisetifolia was inhibited.The type of allelochemicals affected the degree of damage to the roots.Under lower concentration(0–50 mg·L-1)and shorter time(0–36 h)stress,3-O-coffee-acyl-lupeol had the greatest damage to C.equisetifolia and catechin had the minimum damage.Under higher concentration(100–400 mg·L-1)and longer time(48–72 h)stress,12,13-dihydromicromeric acid had the greatest damage and betulinic acid had the minimum damage.

关 键 词：化感胁迫 内生真菌 木麻黄 活性氧含量 保护酶活性 

分 类 号：S792.93[农业科学—林木遗传育种]